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Site-specific bioorthogonal protein labelling by tetrazine ligation using endogenous β-amino acid dienophiles
The tetrazine ligation is an inverse electron-demand Diels–Alder reaction widely used for bioorthogonal modifications due to its versatility, site...
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Application of Restriction Free (RF) Cloning in Circular Permutation
The restriction free (RF) cloning has emerged as one of the highly efficient techniques in the area of genetic engineering. RF cloning has wide range...
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Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep
PTO-QuickStep is a quick and easy molecular cloning technique that allows seamless point integration of a DNA fragment, encoding either a tag or a...
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Cloning and Heterologous Expression of Natural Products from Actinobacteria
Biosynthetic gene clusters (BGCs) are imperative in producing natural products in the microbial world which has been regarded as the most important...
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High-throughput process development from gene cloning to protein production
In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In...
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Restriction-free cloning for molecular manipulation and augmented expression of banana bunchy top viral coat protein
Banana bunchy top virus (BBTV) causing bunchy top disease, is one of the most devastating diseases of banana and plantain. All the six genomic...
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Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1
BackgroundIn this study, we isolated a cellulase-producing bacterium, Bacillus amyloliquefaciens strain elh, from rice peel. We employed two...
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Molecular Cloning, Expression and Enzymatic Characterization of Tetrahymena thermophila Glutathione-S-Transferase Mu 34
Glutathione-S-transferase enzymes (GSTs) are essential components of the phase II detoxification system and protect organisms from oxidative stress...
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Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems
The design and generation of an optimal protein expression construct is the first and essential step in the characterization of any protein of...
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Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
BackgroundThe ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development...
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pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning
DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. In this study, we...
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Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
BackgroundNewcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens...
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Alternative Seamless Cloning Strategies in Fusing Gene Fragments Based on Overlap-PCR
Gene fragment swap** and site-directed mutagenesis are commonly required in dissecting functions of gene domains. While there are many approaches...
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Cloning and Expression of Acetaldehyde Dehydrogenase from Archaea and Its Application in Acrylamide Elimination from Fried Foods
High-temperature frying is a common food processing method, and fried foods are favored by consumers due to their unique flavor and good taste....
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Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli
In this chapter, we present a bidirectional promoter library toolbox to evaluate fast and efficiently the optimal conditions for the balanced...
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Pectinase from a Fish Gut Bacterium, Aeromonas guangheii (SS6): Production, Cloning and Characterization
During the present research, 11 gut bacteria were isolated from the freshwater fish, Systomus sarana (General name: olive barb) and upon screening,...
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CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells
MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of...
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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Gene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological...
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Construction and Assembly of Standardized Biobricks for Synthetic Pathways Engineering in Yeasts
Over the past years, a tremendous variety of modular cloning and DNA assembly strategies has been developed. By definition, they enable one-step...
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DNA synthesis technologies to close the gene writing gap
Synthetic DNA is of increasing demand across many sectors of research and commercial activities. Engineering biology, therapy, data storage and...
