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Generation and Characterization of In Vitro Transcribed mRNA
Here we describe the in vitro preparation of mRNA from DNA templates, including setting up the transcription reaction, mRNA cap**, and mRNA...
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In Vitro Techniques in Plant Breeding
Since the discovery of totipotency in 1902, plant cell and tissue culture has been an integral part of plant biotechnology and crop improvement...
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In Vitro Determination of the Glycemic Index
The use of sourdough as leavening agent, among other nutritional attributes, is preferable for making bread and other leavened baked goods with...
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Progresses in Cell-Free In Vitro Evolution
Biopolymers, such as proteins and RNA, are integral components of living organisms and have evolved through a process of repeated mutation and...
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In Vitro Enzyme-Induced Vinyl Polymerization
The in vitro enzyme-mediated polymerization of vinyl monomers is reviewed with a scope covering enzymatic polymerization of vitamin C...
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In Vitro Techniques for Safeguarding Medicinal Plants with Antiallergic Properties
Medicinal plants possessing antiallergic properties are extensively used in several medicine systems across different countries. However, it is...
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Assaying ATE1 Activity In Vitro
Here, we describe a standard arginyltransferase assay in vitro using bacterially expressed purified ATE1 in a system with a minimal number of...
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In Vitro Determination of Protein Nutritional Indexes
Most of the recent studies investigating the effects of sourdough on baked goods have highlighted its role in improving different nutritional...
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Phenolic Compounds and In Vitro Antioxidant Activity
Phenolic compounds (PCs) are mainly responsible for the antioxidant capacity of bread, which is affected by several factors such as the type of...
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In Vitro Cultures: Challenges and Limitations
Plant tissue culture provides an effective system for large scale production of plants. Several rare and threatened plants have been conserved...
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Improved Sterilization Techniques for Successful In Vitro Micropropagation
Biotechnological solutions based on in vitro plant tissue culture paved the way for assessing desirable features by increasing the efficiency of in...
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In Vitro Phosphatase Assays for the Eya2 Tyrosine Phosphatase
Protein tyrosine phosphatases (PTP), such as the Eyes Absent (Eya) family of proteins, play important roles in diverse biological processes. In vitro...
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Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production
Pentacyclic triterpenoids are a diverse subclass of naturally occurring terpenes with various biological activities and applications. These compounds...
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The Power of Citrus: Antiallergic Activity and In Vitro Propagation Techniques
Fruits from the Citrus genus are broadly demanded for their flavor, nutritional value, and health benefits. Among the various biological activities...
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In Vitro Methodologies for the Safety Assessment of Drugs
Since ages, drugs have been used in some form or the other for treatment of several diseases. Safety assessment of drug is an important step required...
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Preparation of tRNAArg for Arginylation Assay by In Vitro Transcription
This chapter describes the preparation of tRNAArg by in vitro transcription. tRNA produced by this method can be efficiently utilized for in vitro...
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In Vitro Genotoxicity/Mutagenicity Testing of Food Packaging
To ensure the safe use of packaging materials and food contact materials, genotoxicity assessment is one of the requirements of regulatory agencies...
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In Vitro Flowering of Torenia fournieri
Plant cell, tissue, and organ culture techniques along with molecular biology techniques have contributed to providing more knowledge about the...
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In Vitro Studies in Andrographis paniculata Pertaining to Andrographolides Accumulation
Andrographis paniculata, belonging to the family Acanthaceae, is the most valuable Indian medicinal plant. It is widely distributed throughout...
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Study of Tau Liquid-Liquid Phase Separation In Vitro
Aggregation of the microtubule-associated protein tau is one of the major pathogenic events in Alzheimer’s disease and several other...