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Understanding Kinetic Resolution by Hydrolases Maximizing Enantioselectivity
This brief is a concise guide that explores the theory and practical aspects of improving enantioselectivity in enzymatic kinetic resolution, with a...
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Comparative Analysis of the Primary Structures of Glycoside Hydrolases
AbstractFructan-modifying enzymes are divided into fructan-producing enzymes (fructosyl transferases) and fructan hydrolyzing enzymes (invertases,...
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Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases
BackgroundPlant cell walls represent the most plentiful renewable organic resource on earth, but due to their heterogeneity, complex structure and...
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Complete genome sequence of Acinetobacter indicus and identification of the hydrolases provides direct insights into phthalate ester degradation
A strain designated Acinetobacter indicus WMB-7 with the ability to hydrolyze phthalate esters (PAEs) was isolated from the fermented grains of...
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A family of di-glutamate mucin-degrading enzymes that bridges glycan hydrolases and peptidases
Microbes utilize polysaccharides to protect their surfaces and build biofilms, whereas metazoans employ large mucins densely decorated with O -glycans...
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Glycoside hydrolases in the biodegradation of lignocellulosic biomass
Lignocellulose is a plentiful and intricate biomass substance made up of cellulose, hemicellulose, and lignin. Cellulose and hemicellulose are...
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Modularity of Cellulases, Xylanases, and Other Glycosyl Hydrolases Relevant for Biomass Degradation
Cellulases and xylanases are glycoside hydrolases essential for the enzymatic deconstruction of plant polysaccharides across environments and in many...
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CE16 acetylesterases: in silico analysis, catalytic machinery prediction and comparison with related SGNH hydrolases
Bioinformatics analysis was focused on unique acetylesterases annotated in the CAZy database within the CE16 family and simultaneously belonging to...
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Biomass-degrading glycoside hydrolases of archaeal origin
During the last decades, the impact of hyperthermophiles and their enzymes has been intensively investigated for implementation in various...
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Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase
Phthalate acid esters (PAEs), a group of xenobiotic compounds used extensively as plasticizers, have attracted increasing concern for adverse effects...
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A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases
The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of...
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Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron
Serine hydrolases have important roles in signaling and human metabolism, yet little is known about their functions in gut commensal bacteria. Using...
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Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
Use of the 3,5-dinitrosalicylic acid reagent allows the simple, rapid quantification of reducing sugars. The method can be used for analysis of...
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Microbial Conversion of Biomass
Microbial conversion of biomass is a natural process of transformation of biological resources into energy and bioproducts. Lignocellulosic and...
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Understanding Enzymatic Kinetic Resolution
Kinetic resolution involves conversion of one enantiomer more readily to product than the other of the racemic mixture. Desymmetrization, on the...
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Clonal Blumea lacera (Burm. f.) DC. ameliorates diabetic conditions by modulating carbohydrate and lipid hydrolases: a combine in vivo experimental and chemico-biological interaction study
Blumea lacera (Burm. f.) DC. is an aromatic annual herb that has traditionally been used to treat or protect against diabetes. Although it has...
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Microbial Conversion of Biomass
Microbial conversion of biomass is a natural process of transformation of biological resources into energy and bioproducts. Lignocellulosic and...
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Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds
BackgroundThe conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial...
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Isolation and Purification of Organophosphorus Hydrolases Secreted from Acetone-acclimated Phosphorus Accumulating Organisms and Study of Their Properties for Hydrophobic Organophosphorus Sensor
The present work studied an acclimation method for phosphorus accumulating organisms (PAOs) with a high content of acetone in culture solutions to...
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Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a method in which a fluorophore is covalently attached to the reducing end of...