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Enzyme engineering for functional lipids synthesis: recent advance and perspective
Functional lipids, primarily derived through the modification of natural lipids by various processes, are widely acknowledged for their potential to...
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Redesigning Robust Biocatalysts by Engineering Enzyme Microenvironment and Enzyme Immobilization
Enzymes have replaced traditional industrial catalysts as more efficient, eco-friendly, and sustainable alternatives that can be used in different...
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Broadening the Scope of Biocatalysis Engineering by Tailoring Enzyme Microenvironment: A Review
The rational design of catalysts that fine-tune/mimics the enzyme microenvironment remains the subject of supreme interest. Several strategies moving...
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Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
BackgroundClassical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it...
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Engineering heterologous enzyme secretion in Yarrowia lipolytica
BackgroundEukaryotic cells are often preferred for the production of complex enzymes and biopharmaceuticals due to their ability to form...
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Protein Engineering of Lactate Oxidase
AbstractLactate oxidase is a practically important enzyme widely used to detect L-lactate in medical diagnostics and in the food industry. This...
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Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor
Semi-rational enzyme engineering is a powerful method to develop industrial biocatalysts. Profiting from advances in molecular biology and...
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Metabolic Engineering
Metabolic engineering is a powerful methodology aimed at intelligently designing new biological pathways, systems, and ultimately phenotypes...
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Protein Engineering of Bst Polymerase for Isothermal Amplification Purposes
AbstractThis paper reviews the protein engineering of Bst polymerase using various methods. To modify the enzyme, approaches such as the production...
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Loop dynamics and the evolution of enzyme activity
In the early 2000s, Tawfik presented his ‘New View’ on enzyme evolution, highlighting the role of conformational plasticity in expanding the...
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Enzyme Conjugation - A Promising Tool for Bio-catalytic and Biotransformation Applications – A Review
Enzymes have revolutionized conventional industrial catalysts as more efficient, eco-friendly, and sustainable substitutes that can be used in...
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Research progress of multi-enzyme complexes based on the design of scaffold protein
Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to...
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An ITPA Enzyme with Improved Substrate Selectivity
Recent clinical data have identified infant patients with lethal ITPA deficiencies. ITPA is known to modulate ITP concentrations in cells and has a...
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In Vitro Enzyme-Induced Vinyl Polymerization
The in vitro enzyme-mediated polymerization of vinyl monomers is reviewed with a scope covering enzymatic polymerization of vitamin C...
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Engineering an efficient and enantioselective enzyme for the Morita–Baylis–Hillman reaction
The combination of computational design and directed evolution could offer a general strategy to create enzymes with new functions. So far, this...
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A cell engineering approach to enzyme-based fed-batch fermentation
BackgroundA fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities in batch cultures,...
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The reaction mechanism of the Ideonella sakaiensis PETase enzyme
Polyethylene terephthalate (PET), the most abundantly produced polyester plastic, can be depolymerized by the Ideonella sakaiensis PETase enzyme....
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Develo** hydrogels for gene therapy and tissue engineering
Hydrogels are a class of highly absorbent and easily modified polymer materials suitable for use as slow-release carriers for drugs. Gene therapy is...
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Basic Concepts and Applications of Enzyme-Catalyzed Reactions for Biotechnology
Enzyme-catalyzed reactions provide several advantages such as specificity, high catalytic rate, transition state stabilization, etc., over inorganic...