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A strategy for in-house production of a positive selection cloning vector from the commercial pJET1.2/blunt cloning vector at minimal cost
Key messageIn-house production of a positive selection cloning vector could be simple, efficient and low cost.
AbstractDNA cloning technology...
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Megaprimer-Based PCR to Synthesize Fusion Genes for Cloning
Megaprimer-based polymerase chain reaction (PCR) strategies allow the versatile and fast assembly and amplification of a myriad of tailor-made or...
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Label-free cell based impedance measurements of ZnO nanoparticles—human lung cell interaction: a comparison with MTT, NR, Trypan blue and cloning efficiency assays
BackgroundThere is a huge body of literature data on ZnOnanoparticles (ZnO NPs) toxicity. However, the reported results are seen to be increasingly...
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A streamlined strategy for self-production of a commercial positive selection vector, the pJET1.2/blunt cloning vector, using common laboratory E. coli strains
Positive selection vectors carry a lethal gene encoding a toxic product that is harmful to most laboratory E. coli strains. Previously, we reported a...
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Application of Restriction Free (RF) Cloning in Circular Permutation
The restriction free (RF) cloning has emerged as one of the highly efficient techniques in the area of genetic engineering. RF cloning has wide range...
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Optimizing the Metal Bioreduction Process in Recombinant Shewanella azerbaijanica Bacteria: A Novel Approach via mtrC Gene Cloning and Nitrate-Reducing Pathway Destruction
Environmental pollution is growing every day in terms of the increase in population, industrialization, and urbanization. Shewanella azerbaijanica is...
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Cloning and Heterologous Expression of Natural Products from Actinobacteria
Biosynthetic gene clusters (BGCs) are imperative in producing natural products in the microbial world which has been regarded as the most important...
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Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep
PTO-QuickStep is a quick and easy molecular cloning technique that allows seamless point integration of a DNA fragment, encoding either a tag or a...
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High-throughput process development from gene cloning to protein production
In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In...
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Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector
BackgroundDue to its high expression capability, recombination of Escherichia coli and pET vector has become the bioengineering preferred expression...
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Molecular Cloning, Expression and Enzymatic Characterization of Tetrahymena thermophila Glutathione-S-Transferase Mu 34
Glutathione-S-transferase enzymes (GSTs) are essential components of the phase II detoxification system and protect organisms from oxidative stress...
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Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1
BackgroundIn this study, we isolated a cellulase-producing bacterium, Bacillus amyloliquefaciens strain elh, from rice peel. We employed two...
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Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
BackgroundThe ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development...
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Molecular Cloning, Expression, Sequence Characterization and Structural Insight of Bubalus bubalis Growth Hormone-Receptor
The current study focuses on molecular cloning, expression and structural characterization of growth hormone-receptor (GHR) and its extracellular...
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Cloning and characterization of a novel mesophilic xylanase gene Fgxyn3 from Fusarium graminearum Z-1
In order to search for high specific activity and the resistant xylanases to XIP-I and provide more alternative xylanases for industrial production,...
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Design Principles of a Novel Construct for HBB Gene-Editing and Investigation of Its Gene-Targeting Efficiency in HEK293 Cells
Beta-thalassemia is one of the most common monogenic inherited disorders worldwide caused by different mutations in the hemoglobin subunit beta ( HBB )...
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Effect of Domain Manipulation in the Staphylococcal Phage Endolysin, Endo88, on Lytic Efficiency and Host Range
The bacteriophage endolysin Endo88 targeting Staphylococcus aureus PS88 consists of the CHAP and Amidase-2 enzymatic domains and one SH3b targeting...
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A new halotolerant xylanase from Aspergillus clavatus expressed in Escherichia coli with catalytic efficiency improved by site-directed mutagenesis
Daily agro-industrial waste, primarily cellulose, lignin, and hemicellulose, poses a significant environmental challenge. Harnessing...
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Cloning and Functional Characterization of 2-C-methyl-d-erythritol-4-phosphate cytidylyltransferase (LiMCT) Gene in Oriental Lily (Lilium ‘Sorbonne’)
2-C-methyl- d -erythritol-phosphate cytidylyltransferase (MCT) is a key enzyme in the MEP pathway of monoterpene synthesis, catalyzing the generation...
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Cloning, recombinant expression, purification, and functional characterization of AGAAN antibacterial peptide
A recombinant version of the AGAAN antimicrobial peptide (rAGAAN) was cloned, expressed, and purified in this study. Its antibacterial potency and...
