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Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins
Green fluorescent proteins (GFPs) have lightened up almost every aspect of biological research including protein sciences. In the field of membrane... -
Size-Exclusion Chromatography: A Twenty-First Century Perspective
Now in its sixth decade, size-exclusion chromatography (SEC) remains the premier method by which to determine the molar mass averages and...
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Studies of extractable organohalogens in farmed Atlantic salmon muscle and skin by neutron activation in conjunction with size exclusion and solid phase extraction chromatography
An instrumental neutron activation analysis method in conjunction with Compton suppression spectrometry was developed for the simultaneous...
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Modeling study of long-term stability of the monoclonal antibody infliximab and biosimilars using liquid-chromatography–tandem mass spectrometry and size-exclusion chromatography–multi-angle light scattering
Monoclonal antibodies (mAbs) represent a dynamic class of biopharmaceutical products, as evidenced by an increasing number of market authorizations...
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High-Performance Liquid Chromatography
High-performance liquid chromatography (HPLC) is a chromatographic technique of great versatility and analytical power that can be applied to any... -
Methods to Measure NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking
Protein oligomerization is a common principle of regulating cellular responses. Oligomerization of NLRs is essential for the formation of NLR... -
Basic Principles of Chromatography
Chromatography has many applications for analysis of various foods components. It differs from other methods of separation in that a wide variety of... -
Chromatography
Chromatography is among the most promising methods of separation. Since analytical chemistry primarily involves identification and separation of... -
High-Performance Liquid Chromatography (HPLC) Analytical Modelling for Measuring the Onion Anthocyanin Profile
This chapter provides a concise overview of the essay on “High-Performance Liquid Chromatography (HPLC) Analytical Modelling for Measuring the Onion... -
2D Chromatography and 2D Spectroscopy in Analytical Chemistry: an Overview
AbstractThe development of rapid chromatographic procedures is of utmost relevance to analytical laboratories since the modern pharmaceutical...
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Monitoring the Cd2+ release from Cd-containing quantum dots in simulated body fluids by size exclusion chromatography coupled with ICP-MS
Quantification of Cd 2+ release from Cd-containing quantum dots (QDs) is of fundamental importance to elucidate its toxicity to organisms, but remains...
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Gas Chromatography
Gas chromatography (GC) has been used for the determination of a wide range of food components, but it is ideally suited for the analysis of... -
Planar Chromatography as a Method for the Analytical Control of Composite Pharmaceutical Preparations Based on N-Vinylamides
AbstractPlanar chromatography is used to develop methods for the analytical control of processes of the synthesis of biomedical polymers and their...
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Micro simulated moving bed chromatography-mass spectrometry as a continuous on-line process analytical tool
Continuous manufacturing is becoming increasingly important in the (bio-)pharmaceutical industry, as more product can be produced in less time and at...
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Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multiangle Light Scattering
Analytical size-exclusion chromatography (SEC) is a powerful technique that separates proteins based on their hydrodynamic radii. This approach can... -
Specialised Forms of Chromatography
Even though adsorption chromatography is certainly by far the most widely used chromatographic separation method—not least because it is so versatile... -
Estimation of Degree of Hydrolysis of Protein Hydrolysates by Size Exclusion Chromatography
Estimating the degree of hydrolysis (DH) of commercial protein hydrolysates from their molecular weight (MW) median values generated by high...
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Recent Advances in Liquid Chromatography Analysis of Synthetic Polymers
Synthetic polymers are rarely homogeneous chemical species but have multivariate distributions in molecular weight, chemical composition, chain... -
Liquid Chromatography under Critical and Limiting Conditions: A Survey of Experimental Systems for Synthetic Polymers
At the interface between the entropic size exclusion separation and the enthalpydominated liquid adsorption chromatography it is possible,...