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Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens
Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable measure for taxonomic profiling of bacterial communities. This...
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Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System
With the development of nanopore sequencing technology, long reads of DNA sequences can now be determined rapidly from various samples. This protocol...
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Association of Bacterial Communities with Psychedelic Mushroom and Soil as Revealed in 16S rRNA Gene Sequencing
Microbial communities’ resident in the mushroom fruiting body and the soil around it play critical roles in the growth and propagation of the...
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Study on the Bioconversion of Curcumin to Calebin-A Using Spirulina subsalsa and Its Taxonomic Resolution Using 16S rRNA Analysis
Due to morphological convergence and the application of numerous taxonomic concepts, the systematics of filamentous cyanobacteria is still a...
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16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis
Tuberculosis (TB) is an airborne infectious disease caused by Mycobacterium tuberculosis ( M.tb ) whose natural history traces back to 70,000 years. TB...
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Fecal metabonomics combined with 16S rDNA sequencing to analyze the changes of gut microbiota in rats fed with different protein source diets
PurposeWhen blended, animal and plant proteins can complement each other in terms of amino acid composition and release time. In this study, we...
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Whole genome sequencing and phylogenomic analyses of a novel glufosinate-tolerant Pseudomonas species
A novel glufosinate-tolerant Pseudomonas sp. LA21, was isolated from soil samples of an oil palm plantation with a long history of glufosinate...
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Whole-genome sequencing and phylogenomic analyses of a novel zearalenone-degrading Bacillus subtilis B72
Bacillus strain B72 was previously isolated as a novel zearalenone (ZEN) degradation strain from the oil field soil in **njiang, China. The genome of...
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Determination of D-Lactic Acid Production
ProbioticsProbiotics provide several health benefitsHealth benefits and can aid in disease prevention. A safety assessment and logical design of...
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A Nanopore Sequencing Course for Graduate School Curriculum
The high-throughput long-read sequencing has become affordable enough for any molecular biology lab to utilize genome sequencing in their research....
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Developmental stage-associated microbiota profile of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) and their functional prediction using 16S rRNA gene metabarcoding sequencing
The different developmental stage-associated microbiota of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae), was characterized using 16S...
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Identification and multilocus gene characterization of phytoplasmas associated with sweet cherry in India
Symptoms of leaf roll, swollen nodes, flat branch and witches’ broom were observed in five cultivars of sweet cherry from Srinagar, Jammu and Kashmir...
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Identification and taxonomy of Streptomyces justiciae strain RA-WS2: a novel setomimycin producing actinobacterium
The taxonomic position of novel bianthraquinone antibiotic producer Streptomyces strain RA-WS2, a soil isolate from Shivalik region of NW Himalayas,...
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Determination of Biogenic Amine Production
ProbioticsProbiotics are live microorganisms that provide a variety of healthHealth advantages but also have the potential to be toxic. Biogenic...
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Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Nile tilapia, Oreochromis niloticus , is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile...
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First report of Salmonella enterica subsp. enterica serovar Typhimurium in pet reptiles in private household of Makkah, Saudi Arabia
Pet reptiles are gaining popularity among Saudi citizens but owners lack public health-associated awareness. Generally, the pet shops do not properly...
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Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1
BackgroundIn this study, we isolated a cellulase-producing bacterium, Bacillus amyloliquefaciens strain elh, from rice peel. We employed two...
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An innovative molecular approach towards the cost-effective entomological authentication of honey
Honey authentication and traceability are crucial not only for economic purposes but also for ensuring safety. However, the widespread adoption of...
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Characterization of microbiota of naturally fermented sauerkraut by high-throughput sequencing
Sauerkraut is a traditionally fermented cabbage, with a unique taste and beneficial properties, in northeast China. The taste and flavor of sauerkraut ...
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Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from Lactiplantibacillus plantarum WP72/27
We sequenced and described two cryptic plasmids from Lactiplantibacillus plantarum strain WP72/27, termed pLP2.5-11 (OP831909) and pLP3.0-4...
