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Secretion of the cytoplasmic and high molecular weight β-galactosidase of Paenibacillus wynnii with Bacillus subtilis
BackgroundThe gram-positive bacterium Bacillus subtilis is widely used for industrial enzyme production. Its ability to secrete a wide range of...
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SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli
BackgroundIn previous work, we developed an E. coli extracellular secretion platform XTHHly based on the hemolysin A secretion system. It can...
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Plant Platforms for Efficient Heterologous Protein Production
Production of recombinant proteins is primarily established in cultures of mammalian, insect and bacterial cells. Concurrently, concept of using...
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TSPO protein binding partners in bacteria, animals, and plants
The ancient membrane protein TSPO is phylogenetically widespread from archaea and bacteria to insects, vertebrates, plants, and fungi. TSPO’s primary...
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Active secretion of a thermostable transglutaminase variant in Escherichia coli
BackgroundStreptomyces mobaraenesis transglutaminase (smTG) is widely used to generate protein crosslinking or attachment of small molecules....
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Protein Subcellular Localization Prediction
The elucidation of the subcellular localization of proteins is very important in order to deeply understand their functions. In fact, proteins...
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Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei
BackgroundWhile there is growing interest in develo** non-canonical filamentous fungi as hosts for producing secretory proteins, genetic...
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A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica
BackgroundYarrowia lipolytica , a nonconventional oleaginous yeast species, has attracted attention due to its high lipid degradation and...
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Bioprinting Strategies for Secretory Epithelial Organoids
Novel three-dimensional (3D) biofabrication platforms can allow magnetic 3D bioprinting (M3DB) by using magnetic nanoparticles to tag cells and then...
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Protein Secretion Prediction Tools and Extracellular Vesicles Databases
Secreted proteins play important roles in several biological processes such as growth, proliferation differentiation, cell-cell communication,...
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Efficient production and biochemical characterization of a thermostable carboxypeptidase from Bacillus megaterium and its application on flavor improvement of soy isolate protein hydrolysates
A carboxypeptidase M32 (CPM32) gene ( cpm32 ) from Bacillus megaterium was cloned. CPM32 exhibited the highest sequence similarity of 79.3% with the...
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Transcriptome profiling uncovers the involvement of CmXyn1, a glycosyl hydrolase 11, in Cochliobolus miyabeanus pathogenicity
Necrotrophic pathogen Cochliobolus miyabeanus ( C. miyabeanus ) causes rice brown leaf spot disease and drastically affects the yield and quality of...
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Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation
BackgroundThe construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each...
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Synthesis, Molecular Docking Analysis and In vitro Evaluation of Potential Anti-Diabetic Candidates with Harnessing the Effectiveness of Scoparia Dulcis Plant
PurposeNatural products have involved and persistent in playing an essential part for the search of novel entities in the treatment of various...
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Designing of a Chimeric Vaccine Using EIS (Rv2416c) Protein Against Mycobacterium tuberculosis H37Rv: an Immunoinformatics Approach
Mycobacterium tuberculosis (Mtb) is a respiratory pathogen that causes tuberculosis (TB). There are a large number of proteins that are involved in...
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Development of a Novel HTLV-1 Protease: Human Fcγ1 Recombinant Fusion Molecule in the CHO Eukaryotic Expression System
Human T-cell leukaemia virus type 1 (HTLV-1) is the causative agent of two life-threatening diseases, adult T cell leukaemia/lymphoma (ATLL), and...
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Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris
BackgroundProtein synthesis is one of the extremely important anabolic pathways in the yeast expression system Pichia pastoris . Codon optimization...
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Automatic and sensitive detection of West Nile virus non-structural protein 1 with a portable SERS–LFIA detector
A portable surface-enhanced Raman scattering (SERS)–lateral flow immunoassay (LFIA) detector has been developed for the automatic and highly...
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A Novel Continuous Enzyme Coupled Colorimetric Assay for Phospholipase A2 and its Application in the Determination of Catalytic Activity of Oil-Body–Associated Oleosin Protein
The present work reports a novel, continuous, specific, and colorimetric coupled method for the assay of phospholipase A 2 (PLA 2 ), which involves use...
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High-Throughput Detection of Ligand-Protein Binding Using a SplitLuc Cellular Thermal Shift Assay
The confirmation of a small molecule binding to a protein target can be challenging when switching from biochemical assays to physiologically...