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Method to Isolate Dormant Cancer Cells from Heterogeneous Populations
Cancer recurrence is responsible for a high percentage of cancer-related deaths. Primary tumor removal, chemotherapy, and radiotherapy often leave... -
Optimized enantioselective (S)-2-hydroxypropiophenone synthesis by free- and encapsulated-resting cells of Pseudomonas putida
BackgroundAromatic α-hydroxy ketones, such as S -2-hydroxypropiophenone (2-HPP), are highly valuable chiral building blocks useful for the synthesis...
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Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized... -
Characterization of Immobilized Magnetic Fe3O4 Nanoparticles on Raoultella Ornithinolytica sp. and Its Application for Azo Dye Removal
A high-performance immobilized bacterial strain coated with magnetic iron oxide nanoparticles was used for Basic Blue 41 azo dye (BB 41 dye)...
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Immobilization of engineered E. coli cells for asymmetric reduction of methyl acetoacetate to methyl-(R)-3-hydroxybutyrate
The efficient asymmetric bio-synthesis of chiral β -hydroxy esters is of great importance for industrial production. In this work, a simple and...
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Ti4+ functionalized β-cyclodextrin covalent organic framework as a new immobilized metal ion affinity chromatography platform for selective capture of phosphorylated peptides and exosomes
A Ti 4+ functionalized β-cyclodextrin covalent organic framework nanoparticle (named as β-CD-COF@Ti 4+ ) was synthesized using a one-pot method...
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Magnetic Nanoparticles Immobilized Copper(I) Complex: A Novel and Highly Active Catalyst for S-Arylation of Heterocycles
Diaryl sulfides are known as valuable intermediates in the production of various useful compounds, including antibacterial, antifungal, anti-ulcer...
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Photoinactivation of Escherichia coli and Staphylococcus aureus using Porphyrin-CuInS2/ZnS quantum dot conjugates immobilized on Mesoporous Silica
Bacterial pathogens remain major contributors to illnesses as they have developed several resistance mechanisms against standard treatments....
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Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells
Bioproduction of high-value molecules has traditionally been carried out using engineered host cells. However, toxic molecules and membrane proteins... -
Protective Effect of Crocin on Endothelial Cells Integrity: Studied by Surface Plasmon Resonance
Endothelial cell integrity and cell junction disruption are involved in many biological processes such as vascular permeability, progression of...
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of... -
Production of 2-keto-gluconic acid from glucose by immobilized Pseudomonas plecoglossicida resting cells
2-Keto- d -gluconic acid (2KGA) is an important organic acid derived from d -glucose and is used to produce the food antioxidant erythorbic acid. To...
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Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461
Immobilization of microbe cells has been considered as a potential alternative for enhancing biobutanol productivity through acetone-butanol-ethanol...
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Titanium(IV) immobilized affinity chromatography facilitated phosphoproteomics analysis of salivary extracellular vesicles for lung cancer
Extracellular vesicles (EVs) play critical roles in intercellular communications, which contain valuable biomarkers for the detection of cancers....
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Progress in Enzyme-Based Biofuel Cells
Oxidoreductases are highly specific and efficient biocatalysts, that can be applied on electrochemical systems for green energy conversion, such as... -
DNA-empowered synthetic cells as minimalistic life forms
Cells, the fundamental units of life, orchestrate intricate functions — motility, adaptation, replication, communication, and self-organization...
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Green Immobilized Silver Nanoparticles Over Mentha spicata Flower Extract Modified Reduced Graphene Oxide: Investigation of its Antioxidant and Anti-liver Cancer Effects
The current work has been dedicated to the research studies on the development of green formulated nanomedicines to combat the hepatocellular...
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Rapid Purification of Immunoglobulin G Using a Protein A-immobilized Monolithic Spin Column with Hydrophilic Polymers
A rapid purification method was developed for antibody production in Chinese hamster ovary (CHO) cells using a Protein A-immobilized monolithic...
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Enhanced production of ε-poly-l-lysine by immobilized Streptomyces ahygroscopicus through repeated-batch or fed-batch fermentation with in situ product removal
ε-Poly- l -lysine (ε-PL) is a naturally-occurring l -lysine homopolymer having a broad-spectrum antimicrobial activity and used widely as a food...
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Choline oxidase immobilized onto hierarchical porous metal–organic framework: biochemical characterization and ultrasensitive choline bio-sensing
As choline levels can be associated with several diseases such as Alzheimer's, Parkinson’s, and so on, monitoring the choline levels has great...