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A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences
The salivary glands are closely associated with stress conditions and the neuroendocrine system. The concentrations of these salivary biomarkers...
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Paper-based molecularly imprinted-interpenetrating polymer network for on-spot collection and microextraction of dried blood spots for capillary electrophoresis determination of carbamazepine
Carbamazepine is an antiepileptic drug with a narrow therapeutic index, which requires an efficient method for blood level monitoring. Finger-prick...
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Native DIGE for Quantitative and Functional Analysis of Protein Interactomes
Protein–protein interactions and multiprotein assemblies of water-soluble and membrane proteins are inherent features of the proteome, which also...
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Subcellular Fractionation for DIGE-Based Proteomics
Mass spectrometry-based protein methodologies have revolutionized the field of analytical biochemistry and enable the identification of hundreds to...
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Development of paper devices with conductive inks for sulfanilamide electrochemical determination in milk, synthetic urine, and environmental and pharmaceutical samples
This work describes a simple, rapid, and cost-effective method for fabrication of paper-based carbon electrode (US$ <0.01) using conductive ink based...
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Quantitative assessment of AD markers using naked eyes: point-of-care testing with paper-based lateral flow immunoassay
Aβ 42 is one of the most extensively studied blood and Cerebrospinal fluid (CSF) biomarkers for the diagnosis of symptomatic and prodromal Alzheimer’s...
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DIGE Analysis of Animal Tissues
Two-dimensional difference gel electrophoresis (2D-DIGE) is an acrylamide gel electrophoresis-based technique for protein separation and...
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Multicolorimetric ELISA biosensors on a paper/polymer hybrid analytical device for visual point-of-care detection of infection diseases
Enzyme-linked immunosorbent assay (ELISA) is widely used for the detection of disease biomarkers. However, it utilizes time-consuming procedures and...
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Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material
Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult. To...
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LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genoty** and Microsatellite Instability Detection
Microsatellites are short tandem repeats of one to six nucleotides that are highly polymorphic and extensively used as genetic markers in numerous...
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Detecting PTP Protein–Protein Interactions by Fluorescent Immunoprecipitation Analysis (FIPA)
Identifying protein–protein interactions is crucial for revealing protein functions and characterizing cellular processes. Manipulating PPIs has...
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Characterization and Dynamic Analysis of Dissolved Proteins in Lake Biwa Using Two-Dimensional Electrophoresis
Concentrated protein-like fluorophores of dissolved organic matter (DOM) in Lake Biwa were analyzed using sodium dodecyl sulfate-polyacrylamide gel...
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Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological...
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DIGE Analysis of Clinical Specimens
Two-dimensional difference gel electrophoresis (2D-DIGE) is an elegant gel electrophoretic analytical tool for comparative protein assessment. It is...
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2D-DIGE Analysis of Liver Disease in Mice
Hepatocellular carcinoma (HCC) is the major type of primary liver cancer. In this chapter, we describe our routine two-dimensional difference gel...
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Determination of binding constants for strong complexation by affinity capillary electrophoresis: the example of complexes of ester betulin derivatives with (2-hydroxypropyl)-γ-cyclodextrin
Complexation plays an important role in many biological phenomena, the analysis of different samples, optimization of separation processes, and...
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Speciation of chromium in water samples using microfluidic paper-based analytical devices with online oxidation of trivalent chromium
Speciation of chromium (Cr) was demonstrated using microfluidic paper-based analytical devices (μ-PADs) that permit the colorimetric determination of...
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2D-DIGE Proteomic Analysis of Mouse Liver Within 1 Week
Several years have passed since LC (liquid chromatography)-MS (mass spectrometry) became the mainstream for proteomic analysis; however, conventional...
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Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays
Assays for measuring enzyme activity can be useful tools for proteomics applications. Enzyme testing can be performed to validate an experimental...