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Glycoconjugates
Carbohydrates covalently attached to proteins and lipids produce three types of glycoconjugates: proteoglycans, glycoproteins, and glycolipids....
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Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant...
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Analysis of Caenorhabditis Protein Glycosylation
Glycoproteins result from post-translational modification of proteins by glycans attached to certain side chains, with possible heterogeneity due to...
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Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine development
Glycosylation is a key quality attribute that must be closely monitored for protein therapeutics. Established assays such as HILIC-Fld of released...
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Terpolymeric platform with enhanced hydrophilicity via cysteic acid for serum intact glycopeptide analysis
A new polymeric (methyl methacrylate/ethylene glycol dimethacrylate/1,2-epoxy-5-hexene) base/matrix has been fabricated and decorated with...
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A novel hydrophilic polymer-coated magnetic nanomaterial based on the HILIC strategy for fast separation of glycopeptides and glycosylated exosomes
Novel hydrophilic poly( N , N -methylenebisacrylamide/1,2-epoxy-5-hexene) coated magnetic nanospheres functionalized with 2-aminopurine (denoted as Fe 3 O 4 ...
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Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate Cancer
Many clinical biomarkers in cancer are glycoproteins, but the majority of them only consider the protein levels. Indeed, only alfa-fetoprotein (AFP)...
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Composition of the Extracellular Matrix
Following extraction, purification, and in-depth biochemical and functional characterization of a large number of the constituting proteins, it is...
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15N metabolic labeling-TMT multiplexing approach to facilitate the quantitation of glycopeptides derived from cell lines
The study of glycoproteomics presents a set of unique challenges, primarily due to the low abundance of glycopeptides and their intricate...
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Parallel reaction monitoring with multiplex immunoprecipitation of N-glycoproteins in human serum for detection of hepatocellular carcinoma
The N-glycosylation of proteins is one of the most important post-translational modifications relevant to various biological functions. The...
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The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pastoris
BackgroundN-glycosylation is one of the most important post-translational modifications. Many studies have shown that N-glycosylation has a...
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On-tissue amidation of sialic acid with aniline for sensitive imaging of sialylated N-glycans from FFPE tissue sections via MALDI mass spectrometry
Spatial visualization of glycans within clinical tissue samples is critical for discovery of disease-relevant glycan dysregulations. Herein, we...
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Expression and Characterization of a GH38 α-Mannosidase from the Hyperthermophile Pseudothermotoga thermarum
This study focused on the bio-characterization of a GH38 α-mannosidase from the hyperthermophile Pseudothermotoga thermarum DSM 5069. We aimed to...
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Mammalian Antigen Display for Pandemic Countermeasures
Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen...
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Strategies to Enrich, Identify, and Characterize Glycoproteome in Blood Plasma Using Liquid Chromatography High-Resolution Mass Spectrometry
Blood and blood-derived components such as plasma and serum are considered as excellent resources that can be utilized to understand the biology of...
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The opportunity cost of automated glycopeptide analysis: case study profiling the SARS-CoV-2 S glycoprotein
Glycosylation analysis of viral glycoproteins contributes significantly to vaccine design and development. Among other benefits, glycosylation...
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An improved evanescent fluorescence scanner suitable for high-resolution glycome map** of formalin-fixed paraffin-embedded tissue sections
Lectin microarray (LMA) is a high-throughput platform that enables the rapid and sensitive analysis of N- and O- glycans attached to glycoproteins in...
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Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation
Glycoproteins obtained from cell culture supernatants or lysates generally exist as mixtures of over 100 differently glycosylated protein forms...
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Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9
Mammalian cell surface and secreted glycoproteins exhibit remarkable glycan structural diversity that contributes to numerous physiological and...
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Immunostimulatory Properties of Echinacea purpurea and Conservation Strategy
Echinacea purpurea (L.) Moench, a member of the Asteraceae (Compositae) family is an important and well-known medicinal plant. The plant is used in...