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A normalized model based on Taqman real-time PCR assay for quantitative comparison of chicken adulteration in raw and heat-treated hamburgers
Mislabeling of hamburgers with undeclared chicken has frequently been reported. A fast TaqMan real-time PCR assay was developed to estimate chicken...
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Enhancing the Authentication Capability of Triplex Real-Time PCR by Increasing the Primer Specificity
Authenticity identification is crucial for the legal control of meat fraud and adulteration. Authentication capability of a method in mixed meat is...
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Development of a multiplex PCR assay for the simultaneous detection of big blue octopus (Octopus cyanea), giant Pacific octopus (Enteroctopus dofleini), and common octopus (Octopus vulgaris)
Since octopuses are similar in appearance and can be processed into various forms, seafood fraud has been reported. In this study, we developed the...
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Identification and differentiation of Asian seabass and mangrove red snapper fillets by CYTB sequence-based PCR analysis
Overall quality attributes between deskinned Asian seabass and mangrove red snapper fillet are similar. This may lead to the substitution of Asian...
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Real-time PCR for detection of pistachio in food targeting a low copy number gene marker
Pistachio nuts ( Pistacia vera ) are important food allergens. Their correct declaration on food labels is mandated and needs to be checked by...
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Development and validation of multiplex PCR assay for differentiating tunas and billfishes
Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We...
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Marker peptide screening and species-specific authentication of Pheretima using proteomics
Pheretima is a common and valuable animal-derived medication used in traditional Chinese medicine. There are four species of Pheretima specified in...
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Detection of Carica papaya Adulteration in Piper nigrum Using Chloroplast DNA Marker-Based PCR Assays
The spice made from the fruits of Piper nigrum (PN) has economic and medicinal importance. Due to the high demand, as well as export and trade, the...
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Development of highly accurate digital PCR method and reference material for monkeypox virus detection
Human monkeypox has attracted attention recently. Monkeypox virus (MPXV) keeps evolving as it spreading around the world rapidly, which may threaten...
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Interlaboratory study on real-time PCR detection and quantification of the European anglerfish, pike, and seabream parvalbumin gene
This study presents a large-scale interlaboratory comparison (ILC) aimed at detecting and quantifying DNA from two European anglerfish ( Lophius...
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Development of real-time PCR based molecular markers for two medicinal herb Artemisia species A. capillaris and A. iwayomogi
Artemisia capillaris and Artemisia iwayomogi are well-known herbal medicines which are used as hepatotherapeutic drugs. These two herbal species can...
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Direct Reverse Transcription Real-Time PCR of Viral RNA from Saliva Samples Using Hydrogel Microparticles
In recent decades “saliva” has emerged as an important non-invasive biofluid for diagnostic purposes in both human and animal health sectors....
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Self-correction of cycle threshold values by a normal distribution–based process to improve accuracy of quantification in real-time digital PCR
The digital polymerase chain reaction (dPCR) is a new and develo** nucleic acid detection technology with high sensitivity that can realize the...
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Ultra-fast PCR method for the distinguishing between Miichthys miiuy and Sciaenops ocellatus
The mi-iuy croaker Miichthys miiuy has immense commercial value in the Republic of Korea. The red drum Sciaenops ocellatus is widely produced by...
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Assessment of the Real-Time PCR Method Claiming to be Specific for Detection and Quantification of the First Commercialised Genome-Edited Plant
A real-time PCR method was recently published with a claim to be specific for the detection and identification of some genome-edited oilseed rape...
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Development of a rapid detection method for genetically modified rice using the ultra-fast PCR system
Genetically modified (GM) rice varieties containing traits such as tolerance to abiotic stress and resistance against pests and diseases continue to...
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Anti-adhesion Activity of Phenolic Compounds Against Campylobacter jejuni and Listeria monocytogenes Evaluated with PCR-Based Methods
Campylobacter jejuni and Listeria monocytogenes are food-borne zoonotic bacteria with the highest notification rate and the highest death rate in EU,...
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Duplex real-time PCR assay with high-resolution melt analysis for the detection and quantification of Listeria species and Listeria monocytogenes in meat products
Listeria contamination in foods of animal origin is one of the most concerning food safety issues. A duplex, SYBR green-based, real-time PCR assay...
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Molecular Markers for the Detection and Analysis of Somaclonal Variation
A diverse number of molecular techniques are nowadays available to approach the analysis of the genetic stability of plant material regenerated from...
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An Efficient Droplet Digital PCR Approach for Detection DNA at Low Concentrations of Toxigenic Fungi in Food Products
As common toxigenic fungi genera, Aspergillus , Penicillium , and Fusarium , have attracted the attention of governments and people all over the world...
