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The strain level antagonism between chain-forming lacticaseibacillus rhamnosus LV108 and hsryfm 1301
Identifying the interaction between different strains is crucial during the fermentation, clinical trials, production and consumption of mixed...
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Determination of linearized pDNA template in mRNA production process using HPLC
The recent clinical success of messenger RNA (mRNA) technology in managing the Covid pandemic has triggered an unprecedented innovation in production...
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Comparative evaluation of chemically and green synthesized zinc oxide nanoparticles: their in vitro antioxidant, antimicrobial, cytotoxic and anticancer potential towards HepG2 cell line
The major goal of this study was to fabricate ZnO nanoparticles (NPs) via chemical and biological routes to evaluate and compare their physiochemical...
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Insights into the abundance, expression and diversity of key denitrification genes in an ecologically managed greenhouse agricultural soil
Understanding the bacteria associated with nitrification and denitrification is crucial for comprehending the processes that lead to nitrous oxide...
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Silencing circLDLRAD3 Inhibits Lung Cancer Progression by Regulating the miR-497-5p/PFKP Axis
PurposeLung cancer is one of the leading causes of death worldwide. Recent studies have shown that circular RNAs are dysregulated in a variety of...
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miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway
In hepatic fibrosis (HF), hepatic stellate cells (HSCs) form the extracellular matrix (ECM), and the pathological accumulation of ECM in the liver...
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Noncanonical NLRP3 Inflammasome Activation: Standard Protocols
The canonical activation of multimeric inflammasomes usually occurs through caspase-1 activation, and it is characterized by the presence of...
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Recent trends and developments of PCR-based methods for the detection of food-borne Salmonella bacteria and Norovirus
In recent years, rapid detection methods such as polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) have been continuously...
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PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and applications over the last years. Massively parallel...
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Electrochemical Biosensor Based on Single Primer Isothermal Amplification (SPIA) for Sensitive Detection of Salmonella in Food
Salmonella is one of the most common food-borne pathogens, and the food safety problem caused by Salmonella has raised concern globally. In this...
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Hypoxia-Challenged Pancreatic Adenocarcinoma Cell-Derived Exosomal circR3HCC1L Drives Tumor Growth Via Upregulating PKM2 Through Sequestering miR-873-5p
Pancreatic adenocarcinoma (PAAD) is a fatal disease with poor survival. Increasing evidence show that hypoxia-induced exosomes are associated with...
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Gene Downregulation in Potato Roots Using Agrobacterium rhizogenes-Mediated Transformation
Agrobacterium rhizogenes has the ability to transform plant cells by transferring the T-DNA from the Ri plasmid to the plant cell genome. These...
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Deciphering antifungal and antibiofilm mechanisms of isobavachalcone against Cryptococcus neoformans through RNA-seq and functional analyses
Cryptococcus neoformans has been designated as critical fungal pathogens by the World Health Organization, mainly due to limited treatment options...
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Young Sca-1+ bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization
BackgroundPolarization of microglia, the resident retinal immune cells, plays important roles in mediating both injury and repair responses...
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Circ_TEX2 Functions as a Tumor Suppressor in Hepatoma via miR-96-5p/SPRED1 Axis
Circular RNAs (circRNAs) have been shown to have a vital effect on hepatoma progression. The purpose of this study was to explore the function and...
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Deoxyribonucleic Acid Barcoding for the Identification of Botanicals
The Natural Herbal Products industry uses botanicals or herbs as raw materials for production of herbal products or dietary supplements. Recently,...
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The Method of Eliminating the Wolbachia Endosymbiont Genomes from Insect Samples Prior to a Long-Read Sequencing
When extracting DNA of invertebrates for long-read sequencing, not only enough quantity and size of the DNA but, depending on the species,...
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Chondroprotective effects of bone marrow mesenchymal stem cell-derived exosomes in osteoarthritis
Chondrocyte ferroptosis constitutes a major cause of the development of osteoarthritis (OA). Bone marrow mesenchymal stem cell-derived exosomes...
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CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has enabled rapid advances in genomic engineering and transcriptional...
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Circ-C16orf62 Regulates Oxidized low-density Lipoprotein-induced Apoptosis, Inflammation, Oxidative Stress and Cholesterol Accumulation of Macrophages via Mediating RAB22A Expression by Targeting miR-377
Atherosclerosis (AS) is one of the most common and important vascular diseases. It is believed that the abnormal expression of circular RNAs...
