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Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species
BackgroundFuels and chemicals derived from non-fossil sources are needed to lessen human impacts on the environment while providing a healthy and...
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Cloning, expression and characterization of a glycoside hydrolase family 51 α-l-arabinofuranosidase from Thermoanaerobacterium thermosaccharolyticum DSM 571
The present study focused on the characterization of a glycoside hydrolase 51 family α- l -arabinofuranosidase named TtAbf51 from thermophile Thermoanaer...
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Recycle of sugarcane molasses by Aurantiochytrium sp. for high-value docosahexaenoic acid
Sugarcane molasses is one of the by-products from the sugar industry, which is a candidate carbon source for microbes to produce high-value...
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Studying the Role of a Single Mutation of a Family 11 Glycoside Hydrolase Using High-Resolution X-ray Crystallography
XynII is a family 11 glycoside hydrolase that uses the retaining mechanism for catalysis. In the active site, E177 works as the acid/base and E86...
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Enzymatic self-assembly/disassembly turns “ON”/“OFF” the mimetic hydrolytic activity of histidine nanofibers
Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner...
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Transformations of 3-(Bromomethyl)-5,7-dimethyl-2-oxaadamantan-1-ol in Sulfuric Acid
AbstractReactions of 3-(bromomethyl)-5,7-dimethyl-2-oxaadamantan-1-ol with 96% sulfuric acid in the absence and in the presence of nucleophiles have...
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Determination of D-Lactic Acid Production
ProbioticsProbiotics provide several health benefitsHealth benefits and can aid in disease prevention. A safety assessment and logical design of...
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Molecular weight control of poly-γ-glutamic acid reveals novel insights into extracellular polymeric substance synthesis in Bacillus licheniformis
BackgroundThe structural diversity of extracellular polymeric substances produced by microorganisms is attracting particular attention....
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Colorimetric Detection of Acid Phosphatase and Malathion Using NiCo2O4 Nanosheets as Peroxidase-Mimicking Activity
The establishment of a low-cost and facile approach for the assay of acid phosphatase (ACP) and its inhibitor is a research focus. In this paper, we...
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Heterologous expression and characterization of novel GH12 β-glucanase and AA10 lytic polysaccharide monooxygenase from Streptomyces megaspores and their synergistic action in cellulose saccharification
BackgroundThe combination of cellulase and lytic polysaccharide monooxygenase (LPMO) is known to boost enzymatic saccharification of cellulose....
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Genomic Plasticity of Acid-Tolerant Phenotypic Evolution in Acetobacter pasteurianus
Acetic acid bacteria have a remarkable capacity to cope with elevated concentrations of cytotoxic acetic acid in their fermentation environment. In...
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Structure and dynamics analysis of multi-domain putative β-1,4-glucosidase of family 3 glycoside hydrolase (PsGH3) from Pseudopedobacter saltans
Structure and conformational behaviour of a putative β-1,4-glucosidase of glycoside hydrolase family 3 ( Ps GH3) from Pseudopedobacter saltans was...
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Preparation of amino-functionalized silica supports for immobilization of epoxide hydrolase and cutinase: characterization and applications
Mesocellular foam silica (MCF) and Santa Barbara Amorphous (SBA-15) were synthesized using the hydrothermal method and then amino-functionalized to...
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Dependence of Enzymatic Saccharification on the Type of Biomass and Pretreatment
Variety of agricultural crops and other forest residues form an important lignocellulosic biomass feedstock for biorefineries. However, these...
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Targeted-activation superparamagnetic spherical nucleic acid nanomachine for ultrasensitive SERS detection of lysozyme based on a bienzymatic-mediated in situ amplification strategy
Lysozyme (LYS) is a widely used bacteriostatic enzyme. In this paper, we built a sensitive and accurate Raman biosensing platform to detect trace...
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Multi-step biosynthesis of the biodegradable polyester monomer 2-pyrone-4,6-dicarboxylic acid from glucose
Background2-Pyrone-4,6-dicarboxylic acid (PDC), a chemically stable pseudoaromatic dicarboxylic acid, represents a promising building block for the...
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GABA synthesizing lactic acid bacteria and genomic analysis of Levilactobacillus brevis LAB6
This study was conducted to investigate the γ-aminobutyric acid (GABA) production ability of 20 Lactobacillus and 25 Bifidobacterium strains which...
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Beyond Conventional Meat Preservation: Saddling the Control of Bacteriocin and Lactic Acid Bacteria for Clean Label and Functional Meat Products
Advancements in food science and technology have paved the way for the development of natural antimicrobial compounds to ensure the safety and...
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Defecation status, intestinal microbiota, and habitual diet are associated with the fecal bile acid composition: a cross-sectional study in community-dwelling young participants
PurposeBile acid (BA) metabolism by intestinal bacteria is associated with the risk of gastrointestinal diseases; additionally, its control has...
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From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5
With the increase in population growth and environmental pollution, the daily protein supply is facing great challenges. Single-cell protein (SCP)...
