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Real-Time PCR Method for Assessment of ParA-Mediated Recombination Efficiency in Minicircle Production
The in vivo intramolecular recombination of a parental plasmid allows excising prokaryotic backbone from the eukaryotic cassette of interest, leading...
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Development of a Touchdown—Duplex PCR Assay for Authentication of Sheep and Goat Meat
Authenticity in foods of animal origin like meat is an arduous task, since there are various cases of adulteration where morphological identification...
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Establishing DNA markers to differentiate Agastache rugosa and Pogostemon cablin, which are confusedly used as medicinal herbs, using real-time PCR
Agastache rugosa and Pogostemon cablin are used as medicinal herbs and aromatic plants and belong to the family Lamiaceae . Despite differences in...
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Vacuum Oven Drying: A Cost-Effective Way of Producing Field-Deployable Reagents for In-house Real-Time PCR Methods
The polymerase chain reaction (PCR), is a widely used, sensitive and reliable method for detecting pathogens. However, technical limitations may...
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Mixed infection of two mandariviruses identified by high-throughput sequencing in Kinnow mandarin and development of their specific detection using duplex RT-PCR
In the current study, high-throughput sequencing (HTS) was used to identify viruses associated with the Kinnow mandarin ( Citrus reticulata ) plants...
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Establishment and Application of a Multiplex PCR Assay for Detection of Sclerotium rolfsii, Lasiodiplodia theobromae, and Fusarium oxysporum in Peanut
Southern blight, stem rot, and root rot are serious soil-borne fungal diseases of peanut, which are caused by Sclerotium rolfsii , Lasiodiplodia...
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Detection of Toxin Genes by PCR Based Methods
Recently, Bacillus species have gained wide acceptance to be used as probiotics for human consumption. Bacilli are Gram-positive, spore forming...
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PCR-Based Method for Authentication of Meat and Processed Meat from Three Commercially Important Catfish Species
Meat in forms of fresh-cut pieces or fillet or processed meat from broadhead catfish ( Clarias macrocephalus ), African catfish ( Clarias gariepinus ),...
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Detection of porcine DNA in Korean processed foods by real-time PCR
In this study, a commercial DNA extraction kit and a real-time Polymerase Chain Reaction (PCR) kit were applied to detect porcine DNA in Korean...
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Development of molecular markers based on real-time PCR to detect flax and sesame in commercial amaranth products
Amaranthus , Sesamum indicum , and Linum usitatissimum are the most popular oilseed grains worldwide. Protein-rich Amaranthus contains bioactive...
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SCAR marker for identification and discrimination of specific medicinal Lycium chinense Miller from Lycium species from ramp-PCR RAPD fragments
In the current study, ramp-PCR fragments from improved RAPD (random amplified polymorphic DNA) amplification of Lycium ( Goji ) species or cultivars...
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Bioinformatics Research and qRT-PCR Verify Hub Genes and a Transcription Factor-MicroRNA Feedback Network in Intervertebral Disc Degeneration
The present study explores the potentials of bioinformatics analysis to identify hub genes linked to intervertebral disc degeneration (IDD) and...
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Multiplex PCR detection method of genetically modified canola event (MON94100, LBFLFK, and NS-B50027-4) combined with capillary electrophoresis
Genetically modified organisms (GMOs) have been continuously developed for their convenience and productivity. In the past three years, three new GM...
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Detection of Krill in Foods Using Real-Time PCR
FDA has received consumer complaints about food products, most notably sardines, containing krill as an undeclared crustacean shellfish allergen. The...
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Development of real-time PCR method for rapid and accurate detection of Centipedes (Scolopendra mutilans) in food
Centipedes contain pharmacologically active compounds used as important medicinal material. However, the poisons produced by centipedes can cause...
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Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However,...
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Duplex droplet digital PCR (ddPCR) for simultaneous quantification of bovine and porcine gelatin in capsules
Detection of bovine and porcine in gelatin-based products is important as species fraud and product mislabeling may have a detrimental impact on...
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Development of triplex PCR for simultaneous detection of soybean and wheat
There is a constant demand for an effective detection method of major allergens, such as soybean ( Glycine max ) and wheat ( Triticum aestivum ). In this...
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Droplet Digital PCR Assay with Linear Regression Models for Quantification of Buffalo-Derived Materials in Different Food Matrices
Animal origin foods like buffalo meat and milk fat are often used to adulterate high-priced products like mutton/chevon products and cow ghee for...
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The type-III effectors-based multiplex PCR for detection of Xanthomonas campestris pv. campestris causing black rot disease in crucifer crops
The black rot disease in crucifer crops is caused by Xanthomonas campestris pv. campestris (Xcc) which drastically reduces the productivity of crops....
