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Advances in ECM Protein-Based Materials
Proteins are the main building blocks of cell structure that play a vital role in the development of cell skeletons, cell differentiation,...
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Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins
BackgroundKluyveromyces marxianus is a promising cell factory for producing bioethanol and that raised a demand for a high yield of heterologous...
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Enhancement of protein production in Aspergillus niger by engineering the antioxidant defense metabolism
BackgroundResearch on protein production holds significant importance in the advancement of food technology, agriculture, pharmaceuticals, and...
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An Immunoinformatics-Based Study of Mycobacterium tuberculosis Region of Difference-2 Uncharacterized Protein (Rv1987) as a Potential Subunit Vaccine Candidate for Preliminary Ex Vivo Analysis
Mycobacterium tuberculosis (Mtb) is the pathogen that causes tuberculosis and develops resistance to many of the existing drugs. The sole licensed TB...
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Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
Physical cues such as light, heat, or an electrical field can be utilized for traceless, on-demand activation of the expression of a desired...
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Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae
BackgroundXylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive...
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Role of PI(4,5)P2 and Cholesterol in Unconventional Protein Secretion
Besides its protective role in the maintenance of cell homeostasis, the plasma membrane is the site of exchanges between the cell interior and the...
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Modification of N-Linked Glycan Sites in Viral Glycoproteins
Post-translational modification of proteins by the addition of sugar chains, or glycans, is a functionally important hallmark of proteins trafficked...
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Protein overproduction alters exosome secretion in Chinese hamster ovary cells
Despite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chinese hamster ovary (CHO)...
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Structure and Function of Potential Glycosylation Sites of Dynactin-Associated Protein dynAP
Overexpression of human dynactin-associated protein (dynAP) transforms NIH3T3 cells. DynAP is a single-pass transmembrane protein with a...
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Factors affecting protein recovery during Hsp40 affinity profiling
The identification and quantification of misfolded proteins from complex mixtures is important for biological characterization and disease diagnosis,...
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Advances in Synthetic Biology Tools and Engineering of Corynebacterium glutamicum as a Platform Host for Recombinant Protein Production
Corynebacterium glutamicum is a gram-positive and non-pathogenic microorganism that has traditionally been used as a major host for amino acid...
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Protein intake and type 2 diabetes mellitus: an umbrella review of systematic reviews for the evidence-based guideline for protein intake of the German Nutrition Society
PurposeProtein-rich foods show heterogeneous associations with the risk of type 2 diabetes (T2D) and it remains unclear whether habitual protein...
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Bacterial Sterilization and Intracellular Protein Release by a Pulsed Electric Field
Several biotechnological applications of high-voltage pulsed electric field (PEF) are introduced. Electrical breakdown or disruption of a biological...
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A programmable protease-based protein secretion platform for therapeutic applications
Cell-based therapies represent potent enabling technologies in biomedical science. However, current genetic control systems for engineered-cell...
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Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter
BackgroundKomagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and...
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Nature-inspired protein ligation and its applications
The ability to manipulate the chemical composition of proteins and peptides has been central to the development of improved polypeptide-based...
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Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering
BackgroundMost of the proteases classified into the M23 family in the MEROPS database exhibit staphylolytic activity and have potential as...
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Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay
BackgroundBacillus subtilis is one of the workhorses in industrial biotechnology and well known for its secretion potential. Efficient secretion of...
