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Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts
Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be...
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Dark Fermentative Hydrogen Production from Spent Coffee Grounds Hydrolysate by Clostridium butyricum
This study used spent coffee grounds (SCGs) as a substrate for biohydrogen production. Acid hydrolysis of SCGs was conducted under conditions of...
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Entropic-Based Separation of Diastereomers: Size-Exclusion Chromatography with Online Viscometry and Refractometry Detection for Analysis of Blends of Mannose and Galactose Methyl-α-pyranosides at “Ideal” Size-Exclusion Conditions
The separation of carbohydrate diastereomers by an ideal size-exclusion mechanism, i.e., in the absence of enthalpic contributions to the separation,...
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Splenic-targeting biomimetic nanovaccine for elevating protective immunity against virus infection
BackgroundThe prevalence of viral infectious diseases has become a serious threat to public safety, economic and social development. Vaccines have...
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MoO3–catalyzed transformation of corn stalk cellulose to glycolic acid: an experimental and DFT study
In this paper, a green approach is developed for the effective conversion of corn stalk cellulose to glycolic acid with MoO 3 . The effect of different...
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Structure and Antiradical Activity of Hydrogen-Bound Complexes of Protocatechoic Acid with Monosaccharides in Aqueous Media
AbstractIt was found by the photocolorimetry method that binary compositions of protocatechic acid with monosaccharides (galactose, mannose) exhibit...
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Knockdown of SDC-1 Gene Alleviates the Metabolic Pathway for the Development of MODS
This study aims to reveal the metabolic differences between SDC-1 knockout mice and wild-type mice and the metabolic differences caused by shock in...
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Efficient production of 2′-fucosyllactose from fructose through metabolically engineered recombinant Escherichia coli
BackgroundThe biosynthesis of human milk oligosaccharides (HMOs) using several microbial systems has garnered considerable interest for their value...
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Glucose-Binding Dioclea bicolor Lectin (DBL): Purification, Characterization, Structural Analysis, and Antibacterial Properties
In this study, we purified a lectin isolated from the seeds of Dioclea bicolor (DBL) via affinity purification. Electrophoresis analysis revealed...
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Characterization of N-glycome profile in mouse brain tissue regions by MALDI-TOF/MS
Glycosylation is one of the most common types of post-translational modifications in mammals. It is well known that N-glycans play a key role in cell...
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A repertoire of anti-mannan Candida albicans antibodies in the blood sera of healthy donors
Fungal mannans are polysaccharide antigens located on the cell wall surface of various representatives of the fungal kingdom. Titers of the IgG...
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The structure and antioxidant activities of three high molecular weight polysaccharides purified from the bulbs of Lanzhou lily
With hot water extraction and ethanol precipitation, three high molecular weight polysaccharides, LZLs (LZL-1, LZL-2 and LZL-3), were successfully...
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Isolation, Characterization, Antioxidant, and Wound Healing Activities of Extracellular Polysaccharide from Endophytic Fungus Talaromyces purpureogenus
In this study, two extracellular polysaccharides (TEPS1 and TEPS2) were isolated from the endophytic fungus ( Talaromyces purpureogenus ) and purified...
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In silico-guided discovery and in vitro validation of novel sugar-tethered lysinated carbon nanotubes for targeted drug delivery of doxorubicin
ContextMultiwalled carbon nanotubes (MWCNTs) functionalized with lysine via 1,3-dipolar cycloaddition and conjugated to galactose or mannose are...
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An alkali-extracted polysaccharide from Pleurotus eous and exploration of its antioxidant and immunomodulatory activities
The Pleurotus eous fruiting body was used to prepare an alkali-extracted polysaccharide (PEAP). The goal was to characterize PEAP’s physicochemical...
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Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant...
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Characterization, α-Amylase Inhibition and In Silico Docking Study of Polysaccharides Extracted from Rosy Garlic (Allium roseum) Bulbs
This work aimed to extract polysaccharides from Allium roseum bulbs, study and discuss, via in silico analysis, their in vitro inhibitory effects...
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Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase
C -linked glycosylation is essential for the trafficking, folding and function of secretory and transmembrane proteins involved in cellular...
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Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane
A glycoprotein inhibiting the growth of colon cancer cells was isolated from sheep intestinal mucous membrane. The glycoprotein, designated IGP, was...
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Monose-modified organic electrochemical transistors for cell surface glycan analysis via competitive recognition to enzyme-labeled lectin
A competitive strategy for glycan determination on cell surface with organic electrochemical transistors (OECTs) has been developed. The carboxylic...