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Fluidity of insulin action
Unlike the intensive research in pursuit of understanding the molecular mechanisms of insulin signaling and resistance to its biological action...
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Exploring Membrane Microdomains and Functional Protein Clustering in Live Cells with Flow and Image Cytometric Methods
Fluorescence technologies have shown a spectacular spreading in 21st century biological research. This is mainly due to the enormous complexity of...
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Preclinical safety testing of biotechnology-derived pharmaceuticals
The unique and complex nature of biotechnology-derived pharmaceuticals has meant that it is often not possible to follow the conventional safety...
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Array biosensor for detection of toxins
The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and...
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Contribution of the Phosphorylable Complex I in the Growth Phase-Dependent Respiration of C6 Glioma Cells in Vitro
The energy metabolism of rat C6 glioma cells was investigated as a function of the growth phases. Three-dimensional cultures of C6 cells exhibited...
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Neurotransmitter Modulation of Neuronal Calcium Channels
There are many different calcium channels expressed in the mammalian nervous system, but N-type and P/Q-type calcium channels appear to dominate the...
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Probing control of glucose feeding in Vibrio cholerae cultivations
Infection with Vibrio cholerae is a significant problem in many develo** countries. Cultivation of V. cholerae is used in production of cholera...
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Genetically Modified Microorganisms
Over the last 30 years, the ability to modify specific genes in microorganisms has revolutionized numerous fields of the biosciences, including...
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An innovative strategy for immobilization of receptor proteins on to an optical fiber by use of poly(pyrrole–biotin)
We report an innovative and useful procedure for immobilization of antibodies on to a fiber-optic silica surface. The procedure consists in the...
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Expression of Recombinant Proteins via the Plastid Genome
Genetic engineering of plant cells was first accomplished more than 20 years ago. Since this time, nuclear transgenic plants have been used for...
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Lactic Acid Bacteria as Mucosal Delivery Vehicles
The development of effective strategies for the delivery of vaccine antigens to the mucosal tissues has received considerable attention over the past...
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Medical Biotechnology in India
The potential of biotechnology has just began to emerge in the 20th century. After the full knowledge of human genomes is available, biotechnology is...
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Nucleoside Diphosphate Kinases in Mammalian Signal Transduction Systems: Recent Development and Perspective
The role of nucleoside diphosphate (NDP) kinase with special reference to mammalian signal transduction systems was described. The interaction...
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Different individual immune responses elicited by in vitro immunization
We have previously demonstrated that the addition of muramyl dipeptide (MDP), interleukin-2 (IL-2) and IL-4 effectively raises antibody production...
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The development and use of vaccine adjuvants
Interest in vaccine adjuvants is intense and growing, because many of the new subunit vaccine candidates lack sufficient immunogenicity to be...
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Plant Molecular Biology and Biotechnology Research in the Post-Recombinant DNA Era
After the beginning of the recombinant DNA era in the mid-1970s, researchers in India started to make use of the new technology to understand the...
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Mammalian Cell Culture
As discussed in the previous chapters of this book, mammalian cell-culture technology facilitates the production of complex therapeutic proteins...
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Minireview: The NADH: Ubiquinone Oxidoreductase (Complex I) of the Mammalian Respiratory Chain and the cAMP Cascade
Recent work has revealed cAMP-dependent phosphorylation of the 18-kDa IP subunit of the mammalian complex I of the respiratory chain, encoded by the...