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Unraveling the Dynamic Integration of Auxin, Brassinosteroid and Gibberellin in Early Shade-Induced Hypocotyl Elongation
For shade-intolerant plants, a reduction in the red/far-red (R:FR) light ratio signals the close proximity of competitors and triggers...
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Conditional Protein Depletion in the Analysis of Antifungal Drug Resistance in Candida glabrata
This chapter illustrates a method to generate Candida glabrata conditional depletion mutants for SNF2, an ATPase subunit of the SWI/SNF chromatin...
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Coupling Auxin-Inducible Degron System with Ultrastructure Expansion Microscopy to Accelerate the Discovery of Gene Function in Toxoplasma gondii
Ultrastructure expansion microscopy (U-ExM) is an emerging technique allowing the localization of proteins and cellular structures, at a level of...
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Analyses of Genetic Regulation of the Nervous System in the Nematode Caenorhabditis elegans
This chapter aims to provide a comprehensive overview of the methodologies available to dissect genetic regulation of the nervous systems in the...
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The plant-like protein phosphatase PPKL regulates parasite replication and morphology in Toxoplasma gondii
BackgroundThe protozoan parasite Toxoplasma gondii encodes dozens of phosphatases, among which a plant-like phosphatase absent from mammalian...
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Enhancer–promoter contact formation requires RNAPII and antagonizes loop extrusion
Homotypic chromatin interactions and loop extrusion are thought to be the two main drivers of mammalian chromosome folding. Here we tested the role...
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Near-gapless and haplotype-resolved apple genomes provide insights into the genetic basis of rootstock-induced dwarfing
Dwarfing rootstocks have transformed the production of cultivated apples; however, the genetic basis of rootstock-induced dwarfing remains largely...
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MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set
ObjectiveFruit set is triggered after ovule fertilization, as a consequence of the downregulation of ovary growth repressors, such as the tomato...
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Flavin-Containing Monooxygenases (FMO) Genes in Garlic Allium sativum L.: Genome-Wide Identification, Characterization, and Expression Analysis in Response to Fusarium proliferatum
In this study, 39 flavin-containing monooxygenase genes were identified in the genome of garlic ( Allium sativum L.). The distribution of AsFMOs into...
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Expression Analysis of AUX/IAA Family Genes in Apple Under Salt Stress
Members of the auxin/indoleacetic acid (Aux/IAA) gene family in plants are primary auxin-responsive genes that play important roles in many aspects...
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An end-to-end workflow to study newly synthesized mRNA following rapid protein depletion in Saccharomyces cerevisiae
BackgroundGene transcription by RNA polymerase II is a fundamental process in eukaryotic cells. The precise regulation of transcription is necessary...
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Comparative Analysis of Promoters of DREB2B Transcription Factor Genes in Deschampsia antarctica and Other Grasses
AbstractThe organization of promoters of DREB2B TF ortholog genes involved in the response to abiotic stresses was studied in D. antarctica E. Desv....
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The ability of callus tissues induced from three Allium plants to accumulate health-beneficial natural products, S-alk(en)ylcysteine sulfoxides
S -Alk(en)ylcysteine sulfoxides (CSOs), such as methiin, alliin, and isoalliin, are health-beneficial natural products biosynthesized in the genus Allium...
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Effects of gibberellin priming on seedling emergence and transcripts involved in mesocotyl elongation in rice under deep direct-seeding conditions
Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation. The phytohormone gibberellin...
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Identification and Expression Analysis of miR160 and Their Target Genes in Cucumber
miR160 plays a crucial role in various biological processes by regulating their target gene auxin response factor ( ARF ) in plants. However, little is...
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Computational Identification of Citrus reticulata L. microRNAs and the Cis-Acting Regulatory Elements to Predict the Expression Probability of Their Respective MIR Genes
MicroRNA(miRNA), a small non-coding class of RNA that regulates the gene expression, is conserved among several plant species. In the present study,...
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Genome folding principles uncovered in condensin-depleted mitotic chromosomes
During mitosis, condensin activity is thought to interfere with interphase chromatin structures. To investigate genome folding principles in the...
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Creating a Dataset
Each research project starts with formulating a good question. What is not known about your subject, but worth knowing? Once you have formulated a...