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Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing
Single-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional...
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Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
BackgroundSingle-cell RNA-seq has emerged as an innovative technology used to study complex tissues and characterize cell types, states, and lineages...
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Human liver single nucleus and single cell RNA sequencing identify a hepatocellular carcinoma-associated cell-type affecting survival
BackgroundHepatocellular carcinoma (HCC) is a common primary liver cancer with poor overall survival. We hypothesized that there are HCC-associated...
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Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles
BackgroundSingle-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene...
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Single-cell transcriptomics of the ventral posterolateral nucleus-enriched thalamic regions from HSV-1-infected mice reveal a novel microglia/microglia-like transcriptional response
BackgroundMicroglia participate in the immune response upon central nervous system (CNS) infections. However, the role of these cells during herpes...
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A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh...
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Spatiotemporal single-cell analysis of gene expression in the mouse suprachiasmatic nucleus
Mammalian circadian behaviors are orchestrated by the suprachiasmatic nucleus (SCN) in the ventral hypothalamus, but the number of SCN cell types and...
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Large-Scale Single-Nucleus RNA Sequencing Compatible with Complex Archived Samples
Transcriptome profiling at single-cell resolution allows us to identify and assess functional cell types and cellular states, including those within...
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Author Correction: A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Dynamic regulatory elements in single-cell multimodal data implicate key immune cell states enriched for autoimmune disease heritability
In autoimmune diseases such as rheumatoid arthritis, the immune system attacks the body’s own cells. Develo** a precise understanding of the cell...
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Neuronal SNCA transcription during Lewy body formation
Misfolded α-synuclein (α-syn) is believed to contribute to neurodegeneration in Lewy body disease (LBD) based on considerable evidence including a...
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Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy
The human placenta has a vital role in ensuring a successful pregnancy. Despite the growing body of knowledge about its cellular compositions and...
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Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy
Across eukaryotes, genome stability is essential for normal cell function, physiology, and species survival. Aberrant expression of key genes or...
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The Role of Immunocyte Infiltration Regulatory Network Based on hdWGCNA and Single-Cell Bioinformatics Analysis in Intervertebral Disc Degeneration
Immune infiltration plays a crucial role in intervertebral disc degeneration (IDD). In this study, we explored the immune microenvironment of IDD...
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The Olivocerebellar Tract
Neurons in the inferior olive nucleus, the sole origin of cerebellar climbing fibers, project their axons to the cerebellum through the...
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Allele-level visualization of transcription and chromatin by high-throughput imaging
The spatial arrangement of the genome within the nucleus is a pivotal aspect of cellular organization and function with implications for gene...
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Multimodal Nature of the Single-cell Primate Brain Atlas: Morphology, Transcriptome, Electrophysiology, and Connectivity
Primates exhibit complex brain structures that augment cognitive function. The neocortex fulfills high-cognitive functions through billions of...
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Cell subtype-specific effects of genetic variation in the Alzheimer’s disease brain
The relationship between genetic variation and gene expression in brain cell types and subtypes remains understudied. Here, we generated...
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Patch-seq: Advances and Biological Applications
Multimodal analysis of gene-expression patterns, electrophysiological properties, and morphological phenotypes at the single-cell/single-nucleus...
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Single-Cell MTT: A Simple and Sensitive Assay for Determining the Viability and Metabolic Activity of Polyploid Giant Cancer Cells (PGCCs)
Solid tumors and tumor-derived cell lines commonly contain highly enlarged (giant) cancer cells that enter a state of transient dormancy (active...