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Recombinant AAV Purification
Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as...
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Production and Purification of Recombinant Proteins
The growing demand for therapeutic proteins, especially monoclonal antibodies, increases the need for practical and economical processing techniques....
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Expression and Purification of Recombinant ADAMTS8
ADAMTS8 (A Disintegrin-like and Metalloproteinase with Thrombospondin motifs 8) is a secreted zinc-dependent metalloproteinase whose expression is...
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Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
Protein, being a fundamental biological macromolecule, assumes a critical function in various vital activities of cellular growth, heredity,...
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Expression and Purification of Active Monomeric MMP7
MMP7 is the smallest member of the MMP family and plays multiple physiological and pathological roles through interaction with a variety of...
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Purification Process for a Secreted Protein Produced in Insect Cells
The Baculovirus Expression Vector System (BEVS) is used with cultured insect cells to produce a wide variety of heterologous proteins, which can be...
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Purification of Difficult Proteins
Protein expression and purification have been reviewed comprehensively over the years by researchers. Despite the advances in technology made in this...
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Protein Purification by Affinity Chromatography
Affinity chromatography involves targeted purification of biological macromolecules from a crude mixture on the basis of highly specific interaction...
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Isolation and Purification of Human Pancreatic Islets
Successful islet isolation is the key to islet transplantation in diabetic patients. However, islet isolation is a technically complex and...
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Expression and Purification of Mammalian NMDA Receptor Protein for Functional Characterization
N-methyl-D-aspartate (NMDA) receptors are critical for brain function and serve as drug targets for the treatment of neurological and psychiatric...
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Introduction to Recombinant Protein Purification
Purified form of recombinant proteins is a prerequisite for undertaking in vitro biochemical and structural analyses of these macromolecules....
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Purification of MAO A and MAO B from Mammalian Tissue Sources
Procedures are described for the purification of the mitochondrial-bound enzymes human and bovine monoamine oxidases A and B (MAO A and B) from...
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Isolation and Purification of Various Mammalian Cells: Single Cell Isolation
A typical adult mammalian body consists of 30–40 trillion cells. Mammalian cells differ in their shape, size, morphology, and other structural and...
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Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotor
Adeno-associated virus (AAV) vector-based gene therapy is potentially curative for various genetic diseases; however, the development of a scalable...
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Chromatographic Purification of Viruses: State of the Art and Current Trends
Viruses and virus-derived subunits are essential components of pharmaceuticals for vaccinations as well as gene and tumor therapy. For a safe and...
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Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation
ObjectiveOur goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic...
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Nanobody-Targeted Photodynamic Therapy : Nanobody Production and Purification
Nanobodies have recently been introduced to the field of photodynamic therapy (PDT)Photodynamic therapy (PDT) as a very promising strategy to target...
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Back to Old Books Toward Affordable Research: Homemade Phenol-Based Reagent for Triphasic RNA Purification
Covid-19 crisis did hit many socio-economic aspects in the whole world. In the scientific research, the problem is getting even worse, since most of...
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Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA
BackgroundBlood plasma, one of the most studied liquid biopsies, contains various molecules that have biomarker potential for cancer detection,...
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Textbook on Cloning, Expression and Purification of Recombinant Proteins
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA...