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1. Proximity Ligation Assay (PLA)

   Proximity ligation assay (PLA), also referred to as Duolink® PLA technology, permits detection of protein–protein interactions in situ (<40 nm...

   Muhammad S. Alam in Immunohistochemistry and Immunocytochemistry

   Protocol 2022
   

2. Proximity Ligation Assay to Detect the Proximity Between Host Proteins and Viral Proteins of HIV-1

   The study of host-pathogen interaction often requires interrogating the protein-protein interactions and examining post-translational modifications...

   Sutanuka Chakraborty, Shobith Suresh, ... Udaykumar Ranga in HIV Protocols

   Protocol 2024
   

3. In Situ Proximity Ligation Assay to Visualize Protein–Protein Interactions in Tumor Specimens

   Protein–protein interactions (PPI) are the basis of various biological phenomena, such as intracellular signal transduction, gene transcription, and...

   Yasuhiro Miki, Erina Iwabuchi, Takashi Suzuki in Cancer Systems and Integrative Biology

   Protocol 2023
   

4. Proximity ligation assay to detect DUX4 protein in FSHD1 muscle: a pilot study

   Objective

   Aberrant expression in skeletal muscle of DUX4, a double homeobox transcription factor, underlies pathogenesis in facioscapulohumeral...

   Mary Lou Beermann, Sachiko Homma, Jeffrey Boone Miller in BMC Research Notes

   Article Open access 10 May 2022
   

5. Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells

   Proximity ligation in situ assay (PLISA) is a powerful method to quantify endogen protein-protein interactions in cells and simultaneously identify...

   Paul Peixoto, Michaël Guittaut, Eric Hervouet in Apoptosis and Cancer

   Protocol 2022
   

6. Tau-proximity ligation assay reveals extensive previously undetected pathology prior to neurofibrillary tangles in preclinical Alzheimer’s disease

   Background

   Multimerization is a key process in prion-like disorders such as Alzheimer’s disease (AD), since it is a requirement for self-templating...

   Nora Bengoa-Vergniory, Elisavet Velentza-Almpani, ... Javier Alegre-Abarrategui in Acta Neuropathologica Communications

   Article Open access 28 January 2021
   

7. Study of GPCR Homo- and Heteroreceptor Complexes in Specific Neuronal Cell Populations Using the In Situ Proximity Ligation Assay

   Membrane receptor, for example, G-protein-coupled receptors (GPCRs), operates via coordinated changes between the receptor expression, their...

   Manuel Narváez, Minerva Crespo-Ramírez, ... Dasiel O. Borroto-Escuela in Receptor and Ion Channel Detection in the Brain

   Protocol 2021
   

8. PLK1 phosphorylates RhoGDI1 and promotes cancer cell migration and invasion

   Background

   Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1) plays an important role in diverse cellular processes by regulating Rho guanosine...

   Jeewon Lim, Yo Sep Hwang, ... Hee Gu Lee in Cancer Cell International

   Article Open access 14 February 2024
   

9. TDP43 interacts with MLH1 and MSH6 proteins in a DNA damage-inducible manner

   Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that affects the motor neuron. One aspect of the neuropathology involved in...

   Vincent E. Provasek, Manohar Kodavati, ... Muralidhar L. Hegde in Molecular Brain

   Article Open access 05 June 2024
   

10. Proximity Ligation Assay

    Many methods exist to study oncogenic signaling in lymphoma cells, each with certain strengths and weaknesses. The proximity ligation assay (PLA) is...

    Ryan M. Young in Lymphoma

    Protocol 2019
    

11. Discrepancy between distribution of alpha-synuclein oligomers and Lewy-related pathology in Parkinson’s disease

    The pathological hallmarks of Parkinson’s disease (PD) are α-synuclein (αSYN)-positive inclusions referred to as Lewy bodies and Lewy neurites,...

    Hiroaki Sekiya, Asato Tsuji, ... Tatsushi Toda in Acta Neuropathologica Communications

    Article Open access 06 September 2022
    

12. Altered Distribution of SNARE Proteins in Primary Neurons Exposed to Different Alpha-Synuclein Proteoforms

    Growing evidence indicates that the pathological alpha-synuclein (α-syn) aggregation in Parkinson’s disease (PD) and dementia with Lewy bodies (DLB)...

    Emma Brolin, Martin Ingelsson, ... Anna Erlandsson in Cellular and Molecular Neurobiology

    Article Open access 02 May 2023
    

13. Proximity ligation assay reveals both pre- and postsynaptic localization of the APP-processing enzymes ADAM10 and BACE1 in rat and human adult brain

    Background

    Synaptic degeneration and accumulation of amyloid β-peptides (Aβ) are hallmarks of the Alzheimer diseased brain. Aβ is synaptotoxic and...

    Jolanta L. Lundgren, Lina Vandermeulen, ... Susanne Frykman in BMC Neuroscience

    Article Open access 04 February 2020
    

14. The DNA damage sensor ATM kinase interacts with the p53 mRNA and guides the DNA damage response pathway

    Background

    The ATM kinase constitutes a master regulatory hub of DNA damage and activates the p53 response pathway by phosphorylating the MDM2...

    Konstantinos Karakostis, Laurence Malbert-Colas, ... Robin Fåhraeus in Molecular Cancer

    Article Open access 23 January 2024
    

15. Interaction of the Zika virus with the cytoplasmic dynein-1

    Zika virus (ZIKV) infection is a major public health threat, making the study of its biology a matter of great importance. By analyzing the...

    Dan Israel Zavala-Vargas, Giovani Visoso-Carbajal, ... Edgar Morales-Ríos in Virology Journal

    Article Open access 06 March 2023
    

16. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Postmortem Brain

    Protein–protein interactions (PPI) play an important role in cellular functions and biological processes within the organism. G-protein-coupled...

    Marta Valle-León, Víctor Fernández-Dueñas, Francisco Ciruela in Receptor and Ion Channel Detection in the Brain

    Protocol 2021
    

17. Alpha-synuclein oligomers and small nerve fiber pathology in skin are potential biomarkers of Parkinson’s disease

    The proximity ligation assay (PLA) is a specific and sensitive technique for the detection of αSyn oligomers (αSyn-PLA), early and toxic species...

    Elena Vacchi, Camilla Senese, ... Giorgia Melli in npj Parkinson's Disease

    Article Open access 20 December 2021
    

18. Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis

    Background

    Accumulating evidence has demonstrated that aberrant expression of deubiquitinating enzymes is associated with the initiation and...

    Zhitao Han, Qi Jia, ... Chunlei Zhang in Journal of Experimental & Clinical Cancer Research

    Article Open access 04 September 2023
    

19. Identification of Dp140 and α1-syntrophin as novel molecular interactors of the neuronal Ca_V2.1 channel

    The primary function of dystrophin is to form a link between the cytoskeleton and the extracellular matrix. In addition to this crucial structural...

    Margarita Leyva-Leyva, Alejandro Sandoval, ... Ricardo González-Ramírez in Pflügers Archiv - European Journal of Physiology

    Article 25 March 2023
    

20. Kinin B₁ receptor and TLR4 interaction in inflammatory response

    Objective

    We aimed to broaden our understanding of a potential interaction between B1R and TLR4, considering earlier studies suggesting that...

    Carolina Batista, João Victor Roza Cruz, ... João Bosco Pesquero in Inflammation Research

    Article 04 July 2024