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FLIM-FRET Protein-Protein Interaction Assay
Fluorescence lifetime imaging performed under FRET conditions between two interacting molecules is a sensitive and robust way to quantify...
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Protein–Protein Interaction via Two-Hybrid Assay in Yeast
The yeast two-hybrid assay enables detecting interactions between proteins, which makes this tool of particular interest for plant–virus interaction...
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Personalized Protein-Protein Interaction Networks Towards Unraveling the Molecular Mechanisms of Alzheimer’s Disease
Alzheimer’s disease (AD) is a highly heterogenous neurodegenerative disease, and several omic-based datasets were generated in the last decade from...
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Spectroscopic and Molecular Docking Studies of the Interaction of Non-steroidal Anti-inflammatory Drugs with a Carrier Protein: an Interesting Case of Inner Filter Effect and Intensity Enhancement in Protein Fluorescence
Interaction of diclofenac and indomethacin with lysozyme was studied using several spectroscopic and molecular docking methods. Difference UV–visible...
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Protein–protein interaction network-based integration of GWAS and functional data for blood pressure regulation analysis
BackgroundIt is valuable to analyze the genome-wide association studies (GWAS) data for a complex disease phenotype in the context of the...
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Interaction Between SARS-CoV-2 Spike Protein S1 Subunit and Oyster Heat Shock Protein 70
There is growing evidence that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminates the marine environment and is bioaccumulated...
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Progress in mass spectrometry approaches to profiling protein–protein interactions in the studies of the innate immune system
Understanding protein–protein interactions (PPIs) is pivotal for deciphering the intricacies of biological processes. Dysregulation of PPIs underlies...
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Targeting protein interaction networks in mitochondrial dynamics for neurodegenerative diseases
Mitochondria, the powerhouse of eukaryotic cells, are the central organelle controlling energy metabolism and apoptosis. Based on metabolic needs,...
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Exploring gene network and protein interaction analysis of neurotrophin signaling pathway in ameloblastoma
Ameloblastoma is a non-cancerous but aggressive oral tumor emerging from odontogenic epithelial tissue involved during odontogenesis. Since there is...
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Small molecule inhibitor targeting the Hsp70-Bim protein–protein interaction in estrogen receptor-positive breast cancer overcomes tamoxifen resistance
IntroductionEstrogen receptor (ER) positive patients compromise about 70% of breast cancers. Tamoxifen, an antagonist of ERα66 (the classic ER), is...
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Deep-learning-enabled protein–protein interaction analysis for prediction of SARS-CoV-2 infectivity and variant evolution
Host–pathogen interactions and pathogen evolution are underpinned by protein–protein interactions between viral and host proteins. An understanding...
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Interaction Studies of Metal-Based Anticancer Drug Entities with Potential Therapeutic Targets
Interaction studies of small-molecule drug candidates or metal-based drugs with their therapeutic target biomolecules viz., DNA, RNAs, proteins,...
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Protein–protein-interaction assay coupled with proteome profiler and immune activation flow cytometry assay validate immune activation in 3D oral cancer-immune co-culture model
Immune evasion is one of the hallmarks of cancer progression. Immunotherapy failed to produce appreciable treatment response in aggressive oral...
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Prion Protein Conversion and Lipids
The conversion of α-helical rich normal prion protein to a β-sheeted pathogenic isoform is central to prion disease. Decades of studies provided...
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Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair
Proteins are major functional units that are tightly connected to form complex and dynamic networks. These networks enable cells and organisms to...
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Protein–Protein and Protein–RNA Interaction Assays to Determine Similarity of INI1/SMARCB1 and TAR RNA in Binding to HIV-1 Integrase
INI1/SMARCB1 is a host protein that interacts with HIV-1 integrase (IN) and influences multiple stages of viral replication. IN is a viral enzyme...
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Fluorescence Spectroscopic Studies to Evaluate Binding Interaction between Hoechst 33258 and Bilirubin
A detailed spectroscopic study (fluorescence, absorption, and lifetime) was conducted to gain insight into the nature of the binding interaction...
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Pharmacometric model of agalsidase–migalastat interaction in human: a novel mechanistic model of drug-drug interaction between a therapeutic protein and a small molecule
Recently, a new mechanism of drug–drug interaction (DDI) was reported between agalsidase, a therapeutic protein, and migalastat, a small molecule,...
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HIV-1 Vif protein sequence variations in South African people living with HIV and their influence on Vif-APOBEC3G interaction
PurposeDespite extensive research, HIV-1 remains a global epidemic with variations in pathogenesis across regions and subtypes. The Viral Infectivity...
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Protein Purification by Reversed Phase Chromatography and Hydrophobic Interaction Chromatography
With the increasing analysis and studies on purified proteins and peptides with respect to their structure and function, the need for high resolution...