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miRkit: R framework analyzing miRNA PCR array data
ObjectiveThe characterization of microRNAs (miRNA) in recent years is an important advance in the field of gene regulation. To this end, several...
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Cancer Sample Analysis Utilizing Single-Nucleotide Polymorphism Array and Array Comparative Genomic Hybridization
Chromosomal microarray, including single-nucleotide polymorphism (SNP) array and array comparative genomic hybridization (aCGH), enables the...
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Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen
Autoantibodies that recognize self-antigens are believed to have a close relationship with diseases such as autoimmune diseases, cancer, and...
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Multiplex PCR Design for Scalable Resequencing
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable...
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Genoty**, characterization, and imputation of known and novel CYP2A6 structural variants using SNP array data
CYP2A6 metabolically inactivates nicotine. Faster CYP2A6 activity is associated with heavier smoking and higher lung cancer risk. The CYP2A6 gene is...
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A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2
As the worldwide spreading epidemic of SARS-CoV-2, quick inspection and quarantine of passengers for SARS-CoV-2 infection are essential for...
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Digital PCR: modern solution to parasite diagnostics and population trait genetics
The use of polymerase chain reaction (PCR)-based diagnostic approaches has steadily increased in the field of parasitology in recent decades. The...
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Microfluidic PCR and network analysis reveals complex tick-borne pathogen interactions in the tropics
BackgroundIxodid ticks, particularly Rhipicephalus sanguineus s.l., are important vectors of various disease-causing agents in dogs and humans in...
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Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic
BackgroundOver the course of the COVID-19 pandemic, laboratories worldwide have been facing an unprecedented increase in demand for PCR testing...
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Viral Quantification in Bee Samples Using Synthetic DNA Sequences with Real-Time PCR (qPCR)
Pathogen spillover between honey bees and wild pollinators is a relatively new and exciting field of study. It is known that some viral diseases are...
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Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population
HPV16 and 18 are positively correlated with cervical carcinogenesis. However, HPV prevalence tends to vary according to region, nationality, and...
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Simplified detection of the species of origin of antler velvets using single-stranded tag hybridization chromatographic printed-array strip
In this study, we developed a convenient and easy-to-use origin identification method for antler velvets based on a simple DNA extraction technique...
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A multiplex digital PCR assay for detection and quantitation of porcine circovirus type 2 and type 3
Porcine circovirus (PCV) has become a major pathogen, causing major economic losses in the global pig industry, and PCV type 2 (PCV2) and 3 (PCV3)...
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Mitochondrial genome amplification of avian haemosporidian parasites from single-infected wildlife samples using a novel nested PCR approach
Haemosporidian parasites that infect birds (Apicomplexa: Haemosporida) are blood parasites that require an invertebrate host (vector) and a...
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Detection of SMN1 to SMN2 gene conversion events and partial SMN1 gene deletions using array digital PCR
Proximal spinal muscular atrophy (SMA), a leading genetic cause of infant death worldwide, is an early-onset motor neuron disease characterized by...
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A novel tetra-primer ARMS-PCR approach for the molecular karyoty** of chromosomal inversion 2Ru in the main malaria vectors Anopheles gambiae and Anopheles coluzzii
BackgroundChromosomal inversion polymorphisms have been associated with adaptive behavioral, physiological, morphological and life history traits in...
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Multiplex PCR for rapid differential diagnosis of co-prevalent species of Theileria (Theileria annulata and Theileria orientalis) in cattle
Theileriosis is a tick-borne disease that causes enormous losses in the dairy industry. There are several species of Theileria that can infect...
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Droplet digital PCR-based analyses for robust, rapid, and sensitive molecular diagnostics of gliomas
Classification of gliomas involves the combination of histological features with molecular biomarkers to establish an integrated histomolecular...
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The Significance of PCR Primer Design in Genetic Diversity Studies: Exemplified by Recent Research into the Genetic Structure of Marine Species
Genetic markersGenetic markers are widely applied in the study of genetic diversity for many species. The approach incorporates a Polymerase Chain...
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Design of Oligonucleotides for Allele-Specific Amplification Based on PCR and Isothermal Techniques
Single-nucleotide variations have been associated to various genetic diseases, variations on drug efficiency, and differences in cancer prognostics....