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Metagenomic next-generation sequencing of rectal swabs for the surveillance of antimicrobial-resistant organisms on the Illumina Miseq and Oxford MinION platforms
Antimicrobial resistance (AMR) is a public health threat where efficient surveillance is needed to prevent outbreaks. Existing methods for detection...
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Bioinformatic Analysis of Natively Paired VH:VL Antibody Repertoires for Antibody Discovery
Next-generation DNA sequencing (NGS) of human antibody repertoires has been extensively implemented to discover novel antibody drugs, to analyze...
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Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput Sequencing
Repertoire sequencing of B cells is the high-throughput profiling of B cell receptors (BCR) expressed on the surface of B cells and of...
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An Illumina MiSeq-Based Amplicon Sequencing Method for the Detection of Mixed Parasite Infections Using the Blastocystis SSU rRNA Gene as an Example
Parasite mixed infections remain a relatively unexplored field in part due to the difficulties of unraveling complex mixtures of parasite DNA using...
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The Search for and Functional Analysis of Genetic Variants in microRNA-Binding Sites using Massively Parallel Reporter Assay
A large-scale search for the genetic variants with a bias in the representation of alleles in transcriptome data (AE SNPs) and the binding sites in...
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Somatic CAG repeat instability in intermediate alleles of the HTT gene and its potential association with a clinical phenotype
Huntington disease (HD) is a neurodegenerative disorder caused by ≥36 CAGs in the HTT gene. Intermediate alleles (IAs) (27–35 CAGs) are not...
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Development of a simplified and cost-effective sample preparation method for genoty** of human papillomavirus by next-generation sequencing
High-risk human papillomavirus (HPV) infection is the most common cause of cervical cancer, but low-risk HPV strains can sometimes also be involved....
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Genetic polymorphism, constitutive expression and tissue localization of Dirofilaria immitis P-glycoprotein 11: a putative marker of macrocyclic lactone resistance
BackgroundDirofilaria immitis causes dirofilariosis, a potentially fatal condition in canids. Dirofilaria infections can be prevented with a...
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Effects of host species on microbiota composition in Phlebotomus and Lutzomyia sand flies
BackgroundBlood-sucking phlebotomine sand flies are vectors of the protozoan parasites Leishmania spp. Although the intestinal microbiota is...
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Optimal sampling and analysis methods for clinical diagnostics of vaginal microbiome
Next-generation sequencing-based microbiological analysis is a complex way to profile vaginal microbiome samples since each step affects the results...
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Quality Control of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel
Isogenic H/N/KRAS-less mouse embryonic fibroblast (MEF) cell lines have been developed to assist in cell-based assays of RAS inhibitors. The quality...
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Simultaneous targeted amplicon deep sequencing and library preparation for a time and cost-effective universal parasite diagnostic sequencing approach
We recently described a targeted amplicon deep sequencing (TADS) strategy that utilizes a nested PCR targeting the 18S rDNA gene of blood-borne...
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Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies
Next-generation sequencing (NGS) of immunoglobulin (IG) and T cell receptor (TR) rearrangements represents a modern alternative to classical...
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Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues
BackgroundIntratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately...
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Introduction of a multiplex amplicon sequencing assay to quantify DNA methylation in target cytosine markers underlying four selected epigenetic clocks
BackgroundDNA methylation analysis has proven to be a powerful tool for age assessment. However, the implementation of epigenetic age prediction in...
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HER2 mutations in advanced cervical neuroendocrine carcinoma: implications for trastuzumab deruxtecan therapy
Recent clinical evidence shows that the antibody-drug conjugate (ADC) trastuzumab deruxtecan (T-DXd) can successfully treat patients with advanced...
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Effects of Weight Gain During Pregnancy in Normal-Weight Women on Changes in the Gut Microbiota of Pregnant Women in the Third Trimester
With the improvement of living standards and the lack of nutrition awareness during pregnancy, the phenomenon of excessive weight gain (EWG) of...
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Microbial dynamics with CRC progression: a study of the mucosal microbiota at multiple sites in cancers, adenomatous polyps, and healthy controls
Accumulating evidence has related the gut microbiota to colorectal cancer (CRC). Fusobacterium nucleatum has repeatedly been linked to colorectal...
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First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens
Rotavirus A infects many mammalian species, including humans and causes diarrhea and gastrointestinal diseases. The virus also infects various bird...
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MethScore as a new comprehensive DNA methylation-based value refining the prognosis in acute myeloid leukemia
BackgroundChanges in DNA methylation are common events in the pathogenesis of acute myeloid leukemia (AML) and have been repeatedly reported as...
