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Laser capture microdissection as a method for investigating the human hair follicle microbiome reveals region-specific differences in the bacteriome profile
ObjectiveHuman hair follicles (HFs) are populated by a rich and diverse microbiome, traditionally evaluated by methods that inadvertently sample the...
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Proteogenomic characterization of difficult-to-treat breast cancer with tumor cells enriched through laser microdissection
BackgroundBreast cancer (BC) is the most commonly diagnosed cancer and the leading cause of cancer death among women globally. Despite advances,...
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Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues
Laser capture microdissection (LCM) enables researchers to selectively evaluate gene expression profiling of a specific cell type within a tissue....
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Gene Expression Analysis of Laser-Captured Purkinje Cells in the Essential Tremor Cerebellum
Essential tremor (ET) is a common, progressive neurological disease characterized by an 8–12-Hz kinetic tremor. Despite its high prevalence, the...
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Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues
Correlative light-electron microscopy (CLEM) has evolved in the last decades, especially after significant developments in sample preparation,...
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Isolation and Purification of Various Mammalian Cells: Single Cell Isolation
A typical adult mammalian body consists of 30–40 trillion cells. Mammalian cells differ in their shape, size, morphology, and other structural and...
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Rapid assessment of 3-dimensional intra-tumor heterogeneity through cycling temperature capillary electrophoresis
ObjectiveTumors are heterogeneous three-dimensional masses populated by numerous cell types, including distinct sub-clones of cancerous cells....
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LCM-Seq for Retinal Cell Layer-Specific Responses During Optic Nerve Regeneration
LCM-seq is a powerful tool for gene expression analysis from individual or groups of cells that can be spatially isolated. Within the visual system,...
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Spatial proteomics landscape and immune signature analysis of renal sample of lupus nephritis based on laser-captured microsection
ObjectiveWe aimed to reveal a spatial proteomic and immune signature of kidney function regions in lupus nephritis (LN).
Material and methods ...
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Molecular signatures of inherited and acquired sporadic late onset nemaline myopathies
Acquired sporadic late onset nemaline myopathy (SLONM) and inherited nemaline myopathy (iNM) both feature accumulation of nemaline rods in muscle...
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Transcriptomic profiling of early synucleinopathy in rats induced with preformed fibrils
Examination of early phases of synucleinopathy when inclusions are present, but long before neurodegeneration occurs, is critical to both...
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Laser-capture microdissection of murine lung for differential cellular RNA analysis
The lung tissue contains a heterogeneous milieu of bronchioles, epithelial, airway smooth muscle (ASM), alveolar, and immune cell types. Healthy...
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Role of Myeloperoxidase in ROS Generation and Inflammation Response on Prostate Epithelial Cells
Myeloperoxidase (MPO) has been reported in prostate tissue, and considering its pro-oxidant properties, this location might be linked to prostate...
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Effect of muscle fibre types and carnosine levels on the expression of carnosine-related genes in pig skeletal muscle
It is generally accepted that carnosine (β-alanyl- l -histidine) content is higher in glycolytic than in oxidative muscle fibres, but the underlying...
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Single-Cell Proteome Profiling of Neuronal Cells
Characterizing neuronal heterogeneity at the single-cell level can improve our understanding of developmental and disease processes and lead to novel...
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Proteomics of the dentate gyrus reveals semantic dementia specific molecular pathology
Semantic dementia (SD) is a clinical subtype of frontotemporal dementia consistent with the neuropathological diagnosis frontotemporal lobar...
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Comparative analysis of transcriptome remodeling in plaque-associated and plaque-distant microglia during amyloid-β pathology progression in mice
BackgroundResearch in recent years firmly established that microglial cells play an important role in the pathogenesis of Alzheimer's disease (AD)....
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Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids
Many cancers harbor a large fraction of nonmalignant stromal cells intermixed with neoplastic tumor cells. While single-cell transcriptional...
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Somatic copy number variant load in neurons of healthy controls and Alzheimer’s disease patients
The possible role of somatic copy number variations (CNVs) in Alzheimer’s disease (AD) aetiology has been controversial. Although cytogenetic studies...
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Combining the “Sibling Technologies” of Laser Capture Microdissection and Reverse Phase Protein Microarrays
Reverse phase protein microarrays (RPPA) and laser capture microdissection (LCM) are “sibling” technologies that originated from the same laboratory...