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Evaluating the serological status of COVID-19 patients using an indirect immunofluorescent assay, France
An indirect in-house immunofluorescent assay was developed in order to assess the serological status of COVID-19 patients in Marseille, France....
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Primary Antibody Selection and Blocking Techniques for Immunohistochemistry
Immunohistochemical procedures are complex, multiparameter assays that must take into account many factors during protocol development. The overall...
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Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis
Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing...
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Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish
Neurodegenerative diseases and central nervous system (CNS) injuries are frequently characterized by axonal damage, as well as dendritic pathology....
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Comparison of Methods in the Serologic Diagnosis of Cystic Echinococcosis
PurposeCystic echinococcosis (CE) is caused by the larval form of Echinococcus granulosus. Clinical, radiologic, pathologic, and serologic findings...
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The Impact of Dipyridamole on Disease-Associated Microglia Phenotypic Transformation in White Matter Lesions Induced by Chronic Cerebral Hypoperfusion
White matter lesions (WMLs) resulting from chronic cerebral hypoperfusion (CCH) are the leading cause of vascular dementia (VaD). This study aimed to...
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Neither injury induced macrophages within the nerve, nor the environment created by Wallerian degeneration is necessary for enhanced in vivo axon regeneration after peripheral nerve injury
BackgroundSince the 1990s, evidence has accumulated that macrophages promote peripheral nerve regeneration and are required for enhancing...
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Pediatric visceral leishmaniasis: a retrospective study to propose the diagnostic tests algorithm in southern Iran
Leishmania infantum is the most common cause of visceral leishmaniasis (VL) in Iran, where mainly the patients are children under the age of 5 years....
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Camel Contagious Ecthyma
Camel contagious ecthyma (CCE) is a highly contagious viral disease mostly of young camels that contributes to calf debility and occasionally calf...
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Exercise 17: Immunofluorescence Assay (IFA)
Antigens in cells or in tissue sections can be identified by incubating cell smear preparations on glass slides or fixed tissue section with specific...
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Constitutive knockout of interleukin-6 ameliorates memory deficits and entorhinal astrocytosis in the MRL/lpr mouse model of neuropsychiatric lupus
BackgroundNeuropsychiatric lupus (NPSLE) describes the cognitive, memory, and affective emotional burdens faced by many lupus patients. While NPSLE’s...
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In Vitro Models to Study Angiogenesis and Vasculature
The development of vasculature in vivo is an extremely complex process that requires temporal and spatial coordination between multiple cell types to...
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Ultrastructural and phenotypical diversity of macrophages in the rat ileal mucosa
Several types of macrophages have been reported in the intestinal mucosa, but their histological localization remains ambiguous. Here, we obtained...
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CAR T-cell behavior and function revealed by real-time imaging
Adoptive transfer of T-cells expressing chimeric antigen receptors (CAR) has shown remarkable clinical efficacy against advanced B-cell malignancies....
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Use of Antibodies in the Research on Muscarinic Receptor Subtypes
Antibodies can be a powerful tool to detect receptor expression at the protein level. Their main advantage is the potential of good spatial...
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Tailoring 3D Biomaterials for Spinal Cord Injury Repair
Spinal cord injury (SCI), with either traumatic or non-traumatic aetiology, brings lifetime health, economic and social consequences to thousands of...
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YF17D-vectored Ebola vaccine candidate protects mice against lethal surrogate Ebola and yellow fever virus challenge
Ebola virus (EBOV) and related filoviruses such as Sudan virus (SUDV) threaten global public health. Effective filovirus vaccines are available only...
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Laboratory Diagnosis of HEV Infection
Serological and nucleic acid tests for detecting hepatitis E virus (HEV) have been developed for both epidemiologic and diagnostic purposes. The...
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Natural vertical transmission of dengue virus in Latin America and the Caribbean: highlighting its detection limitations and potential significance
Dengue continues to be a major public health concern in Latin America and the Caribbean with many countries in the region having experienced drastic...
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Theranostic Applications of Quantum Dots
Ever since its discovery by Ekimov and Efros in the year 1980s, the interest on quantum dots (QDs) as a category of nanomaterials has increased...