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Gene conversion: a non-Mendelian process integral to meiotic recombination
Meiosis is undoubtedly the mechanism that underpins Mendelian genetics. Meiosis is a specialised, reductional cell division which generates haploid...
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Why Lysogenic Conversion ?
The ability of non-essential prophage genes to modify bacterial phenotype is called lysogenic conversion. Though most notoriously, as well as...
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Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis
By using an inducible site-specific double-strand break (DSB) in budding yeast, it is possible to monitor—in real time—the repair of the break by...
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Gene Editing in Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) can be isolated and collected from the body, genetically modified, and expanded ex vivo. The invention of innovative...
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Dissecting the Immune System through Gene Regulation
The immune system plays a dual role in human health, functioning both as a protector against pathogens and, at times, as a contributor to disease....
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Receptor conversion and survival in breast cancer liver metastases
BackgroundBreast cancer liver metastases (BCLM) is a common cause of breast cancer-related death. The prognostic and predictive value of receptor...
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Gene Editing Tool for Neurodegenerative Diseases
Neurodegenerative diseases (NDs) encompass a heterogeneous group of disorders characterized by the progressive degeneration and death of neural...
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Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation
BackgroundThe emerging concepts of fetal-like reprogramming following tissue injury have been well recognized as an important cue for resolving...
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Evaluation of commercial kits for isolation and bisulfite conversion of circulating cell-free tumor DNA from blood
BackgroundDNA methylation biomarkers in circulating cell-free DNA (cfDNA) have great clinical potential for cancer management. Most methods for DNA...
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A capture methyl-seq protocol with improved efficiency and cost-effectiveness using pre-pooling and enzymatic conversion
ObjectiveThe opportunities for sequencing-based methylome analysis of clinical samples are increasing. To reduce its cost and the amount of genomic...
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Genome-Wide Principles of Gene Regulation
In this chapter, we will discuss about new principles of genome-wide gene regulation as obtained through data from Big Biology projects like ENCODE,...
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Conversion of Astrocyte Cell Lines to Oligodendrocyte Progenitor Cells Using Small Molecules and Transplantation to Animal Model of Multiple Sclerosis
Astrocytes, the most prevalent cells in the central nervous system (CNS), can be transformed into neurons and oligodendrocyte progenitor cells (OPCs)...
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Prion Conversion and Deformed Templating
The transmissible agent of prion disease consists of a prion protein in its abnormal, β-sheet-rich state (PrPSc), which replicates itself according...
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Transgene-free direct conversion of murine fibroblasts into functional muscle stem cells
Transcription factor-based cellular reprogramming provides an attractive approach to produce desired cell types for regenerative medicine purposes....
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Detection of SMN1 to SMN2 gene conversion events and partial SMN1 gene deletions using array digital PCR
Proximal spinal muscular atrophy (SMA), a leading genetic cause of infant death worldwide, is an early-onset motor neuron disease characterized by...
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Phase-Appropriate Potency Assay Development for AAV-Based Gene Therapy Products
The development of any medicinal product requires thorough characterization and quality control to assure drug product potency. Adeno-associated...
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The influence of Nrf2 gene promoter methylation on gene expression and oxidative stress parameters in preeclampsia
Background and aimsPreeclampsia (PE) is a serious medical condition that usually causes high blood pressure and affects multiple organs. Considering...
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Blocked conversion of Lactobacillus johnsonii derived acetate to butyrate mediates copper-induced epithelial barrier damage in a pig model
BackgroundHigh-copper diets have been widely used to promote growth performance of pigs, but excess copper supplementation can also produce negative...
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Gene Therapy Strategies for Prophylactic and Therapeutic Treatments of Human Prion Diseases
Prion disease is a diverse family of fatal and usually transmissible and progressive neurodegenerative diseases that strike humans and many other...
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Gene Therapy of Extracellular Vesicles in Cardiovascular and Metabolic Diseases
The ultimate and most complex form of treating human diseases is embodied by gene therapy. For an effective gene therapeutic product we need to hack...