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Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins
Many negative-sense single-stranded RNA viruses within the order Mononegavirales harm humans. A common feature shared among cells infected by these...
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Characterization of Nuclear HIV-Induced Membraneless Organelles Through Fluorescence Microscopy
The postnuclear entry steps of HIV-1 involve reverse transcription, uncoating, and integration into the host genome. The differential regulation of...
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Profiling Locally Translated mRNAs in Regenerating Axons
Spatial and temporal regulation of protein expression plays important roles in many cellular functions, particularly for highly polarized cell types....
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Construction of Cyan Blue Fluorescence Probe based on Nitrogen-Doped Carbon Dots for Detecting Nitrite Ion in Ham Sausage
Nitrite ion is one of the materials widely used in human life, and the accurate, sensitive and stable detection of nitrite ions is of great...
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Detection of Tyrosinase Activity and Inhibitor Validation Based on N-GQDs Fluorescence Sensor
Tyrosinase inhibitors have the ability to resist melanin formation and can be used for clinical and cosmetic, so it is becoming extremely crucial to...
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A Ratio Fluorescence Method Based on Dual Emissive Copper Nanoclusters for the Detection of Vanillin
In this study, a novel double-emission fluorescence probe at 340 and 400 nm was synthesized by one-pot method using phenylalanine (Phe) and ascorbic...
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Jamun Seed-Derived Nitrogen-Doped Carbon Dots: A Novel Microwave-Assisted Synthesis for Ultra-Bright Fluorescence and Mn7+ Detection
For the synthesis of heteroatom-doped carbon nanostructures, biomass is considered as a promising option. Utilizing the microwave-assisted method, we...
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Structural changes in perineuronal nets and their perforating GABAergic synapses precede motor coordination recovery post stroke
BackgroundStroke remains one of the leading causes of long-term disability worldwide, and the development of effective restorative therapies is...
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Multifocal fluorescence video-rate imaging of centimetre-wide arbitrarily shaped brain surfaces at micrometric resolution
Fluorescence microscopy allows for the high-throughput imaging of cellular activity across brain areas in mammals. However, capturing rapid cellular...
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Smartphone-Aided Fluorescence Detection of Cardiac Biomarker Myoglobin by a Ratiometric Fluorescent AuNCs-QDs Nanohybrids Probe with High Sensitivity
Simple and sensitive detection of cardiac biomarkers is of great significance for early diagnosis and prevention of acute myocardial infarction...
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Using FRAP to Quantify Changes in Transcription Factor Dynamics After Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization
Here we show how to measure the mobility of transcription factors using fluorescence recovery after photobleaching (FRAP). Transcription factors are...
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Laser-Assisted Single-Cell Labeling and Capture
Single-cell technologies have become critical tools to understand and characterize the complex dynamics that govern biological systems, from...
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Carbon Dots with Antioxidant Capacity for Detecting Glucose by Fluorescence and Repairing High-Glucose Damaged Glial Cells
Diabetic mellitus management extends beyond blood glucose monitoring to the essential task of mitigating the overexpression of reactive oxygen...
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Membrane lipid remodeling eradicates Helicobacter pylori by manipulating the cholesteryl 6'-acylglucoside biosynthesis
BackgroundHelicobacter pylori , the main cause of various gastric diseases, infects approximately half of the human population. This pathogen is...
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Development of Simple Fluorescence Analytical Strategy for the Detection of Triazophos Using Greenish-Yellow Emissive Carbon Dots Derived from Curcuma longa
This study describes a new method for synthesizing water-soluble carbon dots (CDs) using “ Curcuma longa ” (green source) named CL-CDs via a...
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Liraglutide provides cardioprotection through the recovery of mitochondrial dysfunction and oxidative stress in aging hearts
Glucagon-like peptide-1 receptor (GLP-1R) agonists improve cardiovascular dysfunction via the pleiotropic effects behind their receptor action....
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Regression Analysis of Confocal FRAP and its Application to Diffusion in Membranes
In most biological processes, diffusion plays a critical role in transferring various bio-molecules to transfer desirable locations in an effective...
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DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids
Fluorescence imaging of nucleic acids is a key technology needed to understand gene expression and the resulting changes in cellular function. This...
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Methyl viologen induced fluorescence quenching of CdTe quantum dots for highly sensitive and selective “off-on” sensing of ascorbic acid through redox reaction
A turn-on fluorescent sensor based on CdTe quantum dots (QDs) is designed for highly sensitive and selective ascorbic acid (AA) detection. CdTe shows...
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Imaging and Sensing Inside the Living Cells. From Seeing to Believing
The full power of fluorescence technique can be demonstrated in imaging and sensing within living cells. Its most important advantage that will...