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Interlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers
State-of-the-art in vitro test systems for nanomaterial toxicity assessment are based on dyes and several staining steps which can be affected by...
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Peroxisome proliferator-activated receptor α agonist induces mouse hepatomegaly through the spatial hepatocyte enlargement and proliferation
Peroxisome proliferator-activated receptor alpha (PPARα) activation-induced hepatomegaly is accompanied by hepatocyte hypertrophy around the central...
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A Quantitative Flow Cytometry –Based Method for Autophagy Detection Across the Cell Cycle
Autophagy is deregulated in cancer cells and often activated as a cellular stress response to anticancer therapies. Flow cytometry–based assays...
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Functionalised Graphene Quantum Dots for Cholesterol Detection in Human Blood Serum
The varied applications of nanotechnology have paved way for several breakthroughs in the realm of biomedical technology. In this challenging era...
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Immunocytochemistry of Primary Cultured Cerebral Cortical Neurons
Immunocytochemistry combined with confocal or superresolution microscopy allows us to observe molecular localization and intracellular structures....
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Generation of A Stable GFP-reporter Zika Virus System for High-throughput Screening of Zika Virus Inhibitors
Zika virus (ZIKV) is associated with severe birth defects and Guillain-Barré syndrome and no approved vaccines or specific therapies to combat ZIKV...
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A Method to Conditionally Measure Target Engagement at Intracellular RAS and RAF Complexes
Dysfunction of the RAS/mitogen-activated protein kinase (MAPK) pathway is a common driver of human cancers. As such, both the master regulator of the...
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Are Compounds Membrane-Associated or Present in the Cytosol? A Study Using Polyphenols in a Colon Carcinoma Cell Line Model
PurposeWe investigated the cytosolic and membrane-associated contents of polyphenols after 4 hours of incubation (50 μM of each polyphenol) in the...
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Chromatin and DNA Dynamics in Mouse Models of Liver Cancers
In recent years, important efforts have been made to understand how the expression of a specific gene repertoire correlates with chromatin...
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Immunoproteasome function maintains oncogenic gene expression in KMT2A-complex driven leukemia
Pharmacologic targeting of chromatin-associated protein complexes has shown significant responses in KMT2A -rearranged ( KMT2A-r ) acute myeloid...
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Molecular, immunological, and physiological evidences of a sphingosine-activated plasma membrane Ca2+-channel in Trypanosoma equiperdum
The hemoparasite Trypanosoma equiperdum belongs to the Trypanozoon subgenus and includes several species that are pathogenic to animals and humans in...
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Induction and Detection of Mitophagy
Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains...
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Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection
Identification of host determinants of coronavirus infection informs mechanisms of viral pathogenesis and can provide new drug targets. Here we...
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Loss of the large conductance calcium-activated potassium channel causes an increase in mitochondrial reactive oxygen species in glioblastoma cells
Mitochondrial potassium (mitoK) channels play an important role in cellular physiology. These channels are expressed in healthy tissues and cancer...
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Measurement of Cytosolic Mitochondrial DNA After NLRP3 Inflammasome Activation
The NLRP3 inflammasome, a key component of the innate immune system that mediates caspase-1 activation, which in turn induces cleavage of the...
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Symmetric inheritance of parental histones contributes to safeguarding the fate of mouse embryonic stem cells during differentiation
Parental histones, the carriers of posttranslational modifications, are deposited evenly onto the replicating DNA of sister chromatids in a process...
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Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF
Catalytic activity of the imitation switch (ISWI) family of remodelers is critical for nucleosomal organization and DNA binding of certain...
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Silybin regulates P450s activity by attenuating endoplasmic reticulum stress in mouse nonalcoholic fatty liver disease
Cytochrome P450s are important phase I metabolic enzymes located on endoplasmic reticulum (ER) involved in the metabolism of endogenous and exogenous...
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The homeobox transcription factor DUXBL controls exit from totipotency
In mice, exit from the totipotent two-cell (2C) stage embryo requires silencing of the 2C-associated transcriptional program. However, the molecular...
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