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DNA Damage and DNA Repair
This chapter describes in more detail the various kinds of DNA damage that can cause mutations and chromosomal alterations as well as the...
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Unconventional activation of PRKDC by TNF-α: deciphering its crucial role in Th1-mediated inflammation beyond DNA repair as part of the DNA-PK complex
BackgroundThe DNA-dependent protein kinase (DNA-PK) complex comprises a catalytic (PRKDC) and two requisite DNA-binding (Ku70/Ku80) subunits. The...
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Expanded usage of the Challenge-Comet assay as a DNA repair biomarker in human populations: protocols for fresh and cryopreserved blood samples, and for different challenge agents
Deficiencies in DNA damage response and repair (DDRR) can cause serious pathological outcomes; therefore, having an ability to determine individual...
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Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay
The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet...
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Comparison of comet-based approaches to assess base excision repair
DNA repair plays an essential role in maintaining genomic stability, and can be assessed by various comet assay-based approaches, including the...
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Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair
The reliance on viral vectors for the production of genetically engineered immune cells for adoptive cellular therapies remains a translational...
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CRISPR-Cas13d screens identify KILR, a breast cancer risk-associated lncRNA that regulates DNA replication and repair
BackgroundLong noncoding RNAs (lncRNAs) have surpassed the number of protein-coding genes, yet the majority have no known function. We previously...
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Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer
BackgroundOvarian cancer patients frequently develop chemotherapy resistance, limiting treatment options. We have previously shown that individuality...
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A Clinical Conundrum with Diagnostic and Therapeutic Challenge: a Tale of Two Disorders in One Case
Living organisms are exposed to exogenous and endogenous agents that affect genomic integrity by creating DNA double strand breaks (DSBs). These...
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Association of DNA repair genes polymorphisms with childhood acute lymphoblastic leukemia: a high-resolution melting analysis
ObjectiveAcute lymphoblastic leukemia (ALL) is one of the most common cancers in children for which the exact pathogenesis is not yet known....
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Excavatolide C/cisplatin combination induces antiproliferation and drives apoptosis and DNA damage in bladder cancer cells
Excavatolide C (EXCC), a marine coral-derived compound, exhibits an antiproliferation effect on bladder cancer cells. The present study evaluated the...
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APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress
BackgroundApurinic/apyrimidinic endonuclease 1 (APE1) imparts radio-resistance by repairing isolated lesions via the base excision repair (BER)...
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Disulfiram enhances the antitumor activity of cisplatin by inhibiting the Fanconi anemia repair pathway
A series of chemotherapeutic drugs that induce DNA damage, such as cisplatin (DDP), are standard clinical treatments for ovarian cancer, testicular...
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New Perspectives on Unscheduled DNA Synthesis: Functional Assay for Global Genomic DNA Nucleotide Excision Repair
The unscheduled DNA synthesis (UDS) assay measures the ability of a cell to perform global genomic nucleotide excision repair (NER). This chapter...
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CBL0137 impairs homologous recombination repair and sensitizes high-grade serous ovarian carcinoma to PARP inhibitors
BackgroundHigh-grade serous ovarian carcinomas (HGSCs) are a heterogeneous subtype of epithelial ovarian cancers and include serous cancers arising...
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DDIAS, DNA damage-induced apoptosis suppressor, is a potential therapeutic target in cancer
Increasing evidence indicates that DNA damage-induced apoptosis suppressor (DDIAS) is an oncogenic protein that is highly expressed in a variety of...
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Dynamic alternative DNA structures in biology and disease
Repetitive elements in the human genome, once considered ‘junk DNA’, are now known to adopt more than a dozen alternative (that is, non-B) DNA...
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MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques
MVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus...
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Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancer
BackgroundImmunotherapy based on checkpoint inhibitors is highly effective in mismatch repair deficient (MMRd) colorectal cancer (CRC). These tumors...
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Impact of indoor air pollution on DNA damage and chromosome stability: a systematic review
Indoor air pollution is becoming a rising public health problem and is largely resulting from the burning of solid fuels and heating in households....
