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    Article

    Enhanced maltotetraose purity in industrial production by a sustainable bio-physical process

    Maltotetraose (G4) consists of four glucose units linked by an α-1,4-glycosidic bond. This compound demonstrates remarkable versatility in food processing and exhibits specific physiological functions, suggest...

    Liyuan Jiang, Haocun Kong, **aofeng Ban in Systems Microbiology and Biomanufacturing (2024)

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    Article

    Engineering starch by enzymatic structure design for versatile applications in food industries: a critical review

    As the second largest production material, starch has important value in textile, food, chemical and other fields. The shortcomings of natural starch can be solved, and its properties can be improved by modify...

    Meng He, Haimin Jiang, Haocun Kong, Caiming Li in Systems Microbiology and Biomanufacturing (2023)

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    Article

    Insights into the thermostability and product specificity of a maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04

    Analyze the thermostability, mode of action, and product specificity of a maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04 (Bst-MFA) from the biochemical and structural point of view.

    **aofang **e, **aofeng Ban, Zhengbiao Gu, Caiming Li, Yan Hong in Biotechnology Letters (2020)

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    Article

    Importance of Trp139 in the product specificity of a maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04

    The maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04 (Bst-MFA) randomly cleaves the α-1,4 glycosidic linkages of starch to produce predominantly maltopentaose and maltohexaose. The thre...

    **aofang **e, Gaoyuan Qiu, Ziqian Zhang in Applied Microbiology and Biotechnology (2019)

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    Article

    Delayed supplementation of glycine enhances extracellular secretion of the recombinant α-cyclodextrin glycosyltransferase in Escherichia coli

    The targeting of recombinant proteins for secretion to the culture medium of Escherichia coli presents significant advantages over cytoplasmic or periplasmic expression. However, a major barrier is inadequate sec...

    Zhaofeng Li, Zhengbiao Gu, Miao Wang, Guocheng Du in Applied Microbiology and Biotechnology (2010)

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    Article

    Mutations at subsite −3 in cyclodextrin glycosyltransferase from Paenibacillus macerans enhancing α-cyclodextrin specificity

    A major disadvantage of cyclodextrin production is the limited cyclodextrin product specificity of cyclodextrin glycosyltransferase (CGTase). Here, we described mutations of Asp372 and Tyr89 at subsite −3 in t...

    Zhaofeng Li, Jiayu Zhang, Miao Wang, Zhengbiao Gu in Applied Microbiology and Biotechnology (2009)

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    Article

    γ-Cyclodextrin: a review on enzymatic production and applications

    Cyclodextrins are cyclic α-1,4-glucans that are produced from starch or starch derivates using cyclodextrin glycosyltransferase (CGTase). The most common forms are α-, β-, and γ-cyclodextrins. This mini-review...

    Zhaofeng Li, Miao Wang, Feng Wang, Zhengbiao Gu in Applied Microbiology and Biotechnology (2007)