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Article
Open AccessDetermining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex
Biological processes are typically actuated by dynamic multi-subunit molecular complexes. However, interactions between subunits, which govern the functions of these complexes, are hard to measure directly. He...
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Chapter and Conference Paper
Design of a Novel Compliant Constant-Force Mechanism with High Lateral Stiffness
Compliant constant-force mechanisms (CCFMs) are widely used in overload protection, surgical manipulation and polishing/deburring to provide a near constant-force output over a range of displacement. This work...
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Article
Open AccessUSP14-regulated allostery of the human proteasome by time-resolved cryo-EM
Proteasomal degradation of ubiquitylated proteins is tightly regulated at multiple levels1–3. A primary regulatory checkpoint is the removal of ubiquitin chains from substrates by the deubiquitylating enzyme ubiq...
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Article
Open AccessDevelopment of a support vector machine learning and smart phone Internet of Things-based architecture for real-time sleep apnea diagnosis
The breathing disorder obstructive sleep apnea syndrome (OSAS) only occurs while asleep. While polysomnography (PSG) represents the premiere standard for diagnosing OSAS, it is quite costly, complicated to use...
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Article
Open AccessMuscle fatigue detection and treatment system driven by internet of things
Internet of things is fast becoming the norm in everyday life, and integrating the Internet into medical treatment, which is increasing day by day, is of high utility to both clinical doctors and patients. Whi...
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Article
Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome
The NLRP3 inflammasome can be activated by stimuli that include nigericin, uric acid crystals, amyloid-β fibrils and extracellular ATP. The mitotic kinase NEK7 licenses the assembly and activation of the NLRP3...
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Article
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. Here we present cryo-electron microscopy structures of the substr...
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Article
Open AccessMagnetic MoS2 pizzas and sandwiches with Mnn (n = 1–4) cluster top**s and fillings: A first-principles investigation
The inorganic layered crystal (ILC) MoS2 in low dimensions is considered as one of the most promising and efficient semiconductors. To enable the magnetism and keep intrinsic crystal structures, we carried out a ...
