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    Open Access

    Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

    Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of C...

    Tomomi Aida, Keiho Chiyo, Takako Usami, Harumi Ishikubo, Risa Imahashi in Genome Biology (2015)