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Article
Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus
We previously reported that the membrane skeletal protein 4.1G in the peripheral nervous system transports membrane palmitoylated protein 6 (MPP6), which interacts with the synaptic scaffolding protein Lin7 an...
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Article
Elucidation of the control mechanism of dynamic tissues using freezing techniques
The preparation of histological specimens from animals and humans is a multi-step process comprising tissue collection, fixation, and dehydration, followed by paraffin embedding. Each process can be achieved u...
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Article
Open AccessPharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock
Hypoxia-inducible factor-1 (HIF-1) plays essential roles in human diseases, though its central role in oxygen homoeostasis hinders the development of direct HIF-1-targeted pharmacological approaches. Here, we ...
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Article
Open AccessMint3 depletion restricts tumor malignancy of pancreatic cancer cells by decreasing SKP2 expression via HIF-1
Pancreatic cancer is one of the most fatal cancers without druggable molecular targets. Hypoxia inducible factor-1 (HIF-1) is a heterodimeric transcriptional factor that promotes malignancy in various cancers ...
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Article
Open AccessEXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells
Cancer cells adapt to various stress conditions by optimizing gene expression profiles via transcriptional and translational regulation. However, whether and how EXOSC9, a component of the RNA exosome complex,...
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Article
Scaffold protein Lin7 family in membrane skeletal protein complex in mouse seminiferous tubules
The membrane skeletal complex, protein 4.1G–membrane palmitoylated protein 6 (MPP6), is localized in spermatogonia and early spermatocytes of mouse seminiferous tubules. In this study, we investigated the Lin7...
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Article
The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system
A membrane skeletal molecular complex, protein 4.1G–membrane palmitoylated protein 6 (MPP6)–Lin7–cell adhesion molecule 4 (CADM4), is incorporated in Schwann cells, especially in Schmidt–Lanterman incisures (S...
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Chapter
Structures and Molecular Composition of Schmidt–Lanterman Incisures
Schmidt–Lanterman incisure (SLI) is a circular-truncated cone shape in the myelin internode that is a specific feature of myelinated nerve fibers formed in Schwann cells in the peripheral nervous system (PNS)....
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Article
Deficiency of a membrane skeletal protein, 4.1G, results in myelin abnormalities in the peripheral nervous system
We previously demonstrated that a membrane skeletal molecular complex, 4.1G–membrane palmitoylated protein 6 (MPP6)–cell adhesion molecule 4, is incorporated in Schwann cells in the peripheral nervous system (...
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Article
Open AccessPolymorphic regulation of mitochondrial fission and fusion modifies phenotypes of microglia in neuroinflammation
Microglia are the resident macrophages of the central nervous system and play complex roles in the milieu of diseases including the primary diseases of myelin. Although mitochondria are critical for cellular f...
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Article
Renaissance of morphological studies: the examination of functional structures in living animal organs using the in vivo cryotechnique
Medical and biological scientists wish to understand the in vivo structures of the cells and tissues that make up living animal organs, as well as the locations of their molecular components. Recently, the liv...
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Article
Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy
Serial block-face imaging using scanning electron microscopy enables rapid observations of three-dimensional ultrastructures in a large volume of biological specimens. However, such imaging usually requires da...
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Article
Open AccessConductive resins improve charging and resolution of acquired images in electron microscopic volume imaging
Recent advances in serial block-face imaging using scanning electron microscopy (SEM) have enabled the rapid and efficient acquisition of 3-dimensional (3D) ultrastructural information from a large volume of b...
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Article
Involvement of membrane skeletal molecules in the Schmidt–Lanterman incisure in Schwann cells
Membrane skeletal networks form a two-dimensional lattice structure beneath erythrocyte membranes. 4.1R-MPP (membrane palmitoylated protein) 1-glycophorin C is one of the basic molecular complexes of the membr...
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Chapter
Immunolocalization of Phospho-Arg-Directed Protein Kinase-Substrate in Hypoxic Kidneys
Protein kinases (PKs) phosphorylate proteins at active regions for signal transduction. In this study, normal and hypoxic mouse kidneys were prepared using an “in vivo cryotechnique” (IVCT) and examined immuno...
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Chapter
Function of Dynamin-2 in the Formation of Discoid Vesicles in Urinary Bladder Umbrella Cells
Dynamin (Dyn) is a GTPase that promotes endocytic processes through scission of cell membranes. In this chapter, we present the contribution of Dyn2 to the structural integrity of discoid vesicles (DVs) and to...
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Chapter
Immunohistochemical Detection of Angiotensin II Receptors in Mouse Cerebellum
Angiotensin II (AT) receptors, including AT receptor type 1 (AT1R) and type 2 (AT2R), are expressed in the rodent central nervous system, but their distributions and activation states are still unclear. In thi...
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Chapter
Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Phosphorylated Rhodopsin in Light-Exposed Retina of Living Mouse
In this chapter, we present time-dependent molecular states of rhodopsin (Rho) phosphorylation in mouse retina with “in vivo cryotechnique” (IVCT). Whole eyeballs of living mice under various dark- and light-e...
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Chapter
Three-Dimensional Reconstruction of Liver Tissues with Confocal Laser Scanning Microscopy
Soluble proteins and glycogen particles are well preserved in paraffin-embedded sections prepared by in vivo cryotechnique (IVCT) followed by freeze-substitution (FS) fixation. We performed confocal laser scan...
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Chapter
Application of “In Vivo Cryotechnique” to Immunohistochemical Study of Mouse Sciatic Nerves Under Various Stretching Conditions
In this chapter, we present dynamic structures of peripheral nerve fibers under various stretching conditions and distribution of soluble serum proteins with “in vivo cryotechnique” (IVCT). The structures of I...
