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Article
Chemiluminescence Method for the Determination of Glutathione in Human Serum Using the Ru(phen)3 2+ – KMnO4 System
A simple, rapid and specific chemiluminescence (CL) method for the determination of glutathione has been developed. The method is based on the enhanced CL of the reaction between Ru(phen)3 2+ (phe...
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Article
Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe
A new ligand, 2-phenyl(5-fluoro)imidazo[f]-l,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and D...
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Article
Dot-immunobinding assay using indirect photoacoustic measurement
Dot-immunobinding assay of hepatitis B surface antigen (HBsAg) was performed by a conventionally dot-ELISA technique with 4-chloro-1-naphthol staining, and the quantitative results were measured by an indirec...
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Article
Determination of DNA by Use of the Molecular “Light Switch” Complex of Ru(bipy)2(dppz)2+
A novel fluorimetric method has been developed for selective determination of DNA with the molecular “Light Switch” complex of Ru(bipy)2(dppz)2+. The maximum fluorescence intensity was produced in the pH range o...
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Article
Chemiluminescence Method for the Determination of DNA Using the Ru(bipy)3 2+-Ce(IV) System
A direct chemiluminescence method for the determination of DNA has been developed. The method is based on the chemiluminescence light produced by the reaction of DNA, Ru(bipy)3 2+(bipy= 2,2′-bipydyl) and Ce(IV) ...
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Article
Development of a Direct Chemiluminescence Method for the Determination of Nucleic Acids Based upon Their Reaction with Cerium(IV) in the Presence of Rutheniumtrisdipyridine
A direct chemiluminescent method for the determination of nucleic acids has been developed. The method is based on the enhancement of nucleic acids on the chemiluminescence light emission of the reaction betwe...
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Article
A direct chemiluminescence method for the determination of nucleic acids using Ru(phen)32+–Ce(IV) system
A direct chemiluminescence method for the determination of nucleic acids has been developed based on the enhancement of nucleic acids on the chemiluminescence light emission of the reaction between Ru(phen)3 ...
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Article
Chemiluminescence Determination of Tetracyclines Using a Tris(2,2′-bipyridine)ruthenium(II) and Potassium Permanganate System
A chemiluminescence method for the determination of tetracyclines was developed. The method is based on an enhancement of tetracyclines upon the chemiluminescence light emission of tris(2,2′-bipyridine)rutheni...
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Article
Chemiluminescence Determination of Thiourea Using Tris(2,2′-bipyridyl)ruthenium(II)–KMnO4 System
The emission produced by thiourea in oxidation by permanganate in acidic solution in the presence of Ru(bipy)32+ is used to determine 1.8×10–8 to 1.8×10–6 mol/l thiourea. The limit of detection is 1.0×10–8 mol/l ...
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Article
Measurements of the DNA Content in a Breast Tumor Cell Based on the Hadamard Transform Microscopic Fluorescence Image
The technique of Hadamard transform microscopic fluorescence imaging is described. With an Acridine Orange (AO) staining cell, some factors that influence the fluorescence intensity of a cellular AO-DNA comple...
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Article
Chemiluminescence determination of sulfite in sugar and of sulfur dioxide in air using the tris(2,2′-bipyridyl)ruthenium-KIO4 system
The chemiluminescence (CL) detection for the determination of sulfite using the reaction of Ru(bipy)3 2+(bipy=2,2′-bipyridyl)-SO3 2–-KIO4 is described. The conce...
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Article
High-Performance Liquid Chromatographic Determination of Oxalic Acid in Tea Using Tris(1,10-phenanthroline)-ruthenium(II) Chemiluminescence
The chemiluminescence (CL) of Ru(phen)32+ was applied to HPLC determination of oxalic acid, which was separated by a C18 reverse-phase column with a mobile phase of 0.02 mol/1 NH4Ac, mixed with 0.25 mol/1 Ru(phen...
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Article
Chemiluminescence Determination of Sulfite in Sugar and Sulfur Dioxide in Air Using Ru(bipy)32+-K2S2O8 System
A chemiluminescence (CL) detection method for the determination of sulfite is described. The light produced in the reaction of Ru(bipy)32+(bipy=2,2′-bipyridyl)-SO32--K2S2O8 is detected. The concentration of sulfi...
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Article
The analysis of DNA and protein in a single cell by Hadamard transform microscope image
The Hadamard transform microscope image analysis was applied to analyze the DNA and protein in a single cell. By this method, the fluorescence emission spectrum, the fluorescence image and the fluorescence in...
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Article
Chemiluminescent Determination of 6-Mercaptopurine in Pharmaceutical Preparation
A new chemiluminescence method is described for the determination of 6-mercaptopurine. In the presence of tris(2,2’-bipyridine)ruthenium(II), 6-mercaptopurine (6MP) and hydrogen peroxide, upon the addition of ...
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Article
Application of the synergetic luminescence of Eu in time-resolved fluoroimmunoassay
The synergetic luminescence system of europium for time-resolved fluoroimmunoassay has been studied for the first time. The europium label is bound to the antibody with diethylenetriaminepentaacetic acid anhyd...
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Article
Speciation of Trace Mercury in Natural Water by Reversed-Phase High-Performance Liquid Chromatography
Using dithizone as a precolumn derivatization reagent, four species of Hg were quantitatively separated and determined by RP-HPLC. The method described has high analytical sensitivity, it can be applied for sp...
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Article
A New Method for Determination of Rare Earth Elements Vaporized in Graphite Furnace with a Polytetrafluoroethylene Slurry Fluorinating Reagent by Inductively Coupled Plasma Atomic Emission Spectrometry
A new method based on fluorinating vaporization from a graphite furnace for ICP-AES by using a polytetrafluoroethylene slurry as a fluorinating reagent has been established to determine rare earth elements whi...
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Article
Slurry sample introduction with fluorinating electrothermal vaporization for the direct ICP-AES determination of boron in plant leaves
The slurry sample introduction with fluorinating electrothermal vaporization has been applied to ICP-AES for the determination of boron in plant leaves. The main working parameters for ETV-ICP-AES, such as r.f...
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Article
Fluorination Assisted Electrothermal Vaporization-Inductively Coupled Plasma Atomic Emission Spectrometry for a Direct Determination of Chromium in Biological Materials
Polytetrafluoroethylene (PTFE) was used as a fluorinating agent in order to facilitate a direct analysis of Cr in biological materials by electrothermal vaporization-inductively coupled plasma atomic emission ...
