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Article
Open AccessCDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockade
Serine/threonine kinase, cell division cycle 7 (CDC7) is critical for initiating DNA replication. TAK-931 is a specific CDC7 inhibitor, which is a next-generation replication stress (RS) inducer. This study pr...
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Article
Open AccessComparative pathogenicity of SARS-CoV-2 Omicron subvariants including BA.1, BA.2, and BA.5
The unremitting emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants necessitates ongoing control measures. Given its rapid spread, the new Omicron subvariant BA.5 requires urgent...
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Article
Open AccessMultimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan
SARS-CoV-2 continues to spread worldwide. Patients with COVID-19 show distinct clinical symptoms. Although many studies have reported various causes for the diversity of symptoms, the underlying mechanisms are...
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Article
Open AccessSingle-cell and spatial analyses of cancer cells: toward elucidating the molecular mechanisms of clonal evolution and drug resistance acquisition
Even within a single type of cancer, cells of various types exist and play interrelated roles. Each of the individual cells resides in a distinct microenvironment and behaves differently. Such heterogeneity is...
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Article
Open AccessPotentiality of multiple modalities for single-cell analyses to evaluate the tumor microenvironment in clinical specimens
Single-cell level analysis is powerful tool to assess the heterogeneity of cellular components in tumor microenvironments (TME). In this study, we investigated immune-profiles using the single-cell analyses of...
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Article
Open AccessCombination treatment with a PI3K/Akt/mTOR pathway inhibitor overcomes resistance to anti-HER2 therapy in PIK3CA-mutant HER2-positive breast cancer cells
Amplification and/or overexpression of human epidermal growth factor receptor 2 (HER2) are observed in 15–20% of breast cancers (HER2+ breast cancers), and anti-HER2 therapies have significantly improved progn...
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Article
Open AccessSingle-cell sequencing techniques from individual to multiomics analyses
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and examples of their ...
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Chapter
An Informative Approach to Single-Cell Sequencing Analysis
Recent advances in sequencing technologies enable us to obtain genome, epigenome and transcriptome data in individual cells. In this review, we describe various platforms for single-cell sequencing analysis ac...
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Article
Open AccessCombinatory use of distinct single-cell RNA-seq analytical platforms reveals the heterogeneous transcriptome response
Single-cell RNA-seq is a powerful tool for revealing heterogeneity in cancer cells. However, each of the current single-cell RNA-seq platforms has inherent advantages and disadvantages. Here, we show that comb...
