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Protocol
NanoIndentation, an ImageJ Plugin for the Quantification of Cell Mechanics
Growth and morphogenesis in plants depend on cell wall mechanics and on turgor pressure. Nanoindentation methods, such as atomic force microscopy (AFM), enable measurements of mechanical properties of a tissue...
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Protocol
Visualizing Protein Associations in Living Arabidopsis Embryo
Protein–protein interactions (PPI) are essential for a plethora of biological processes. These interactions can be visualized and quantified with spatial resolution using Förster resonance energy transfer (FRE...
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Article
In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots
Imaging in living Arabidopsis roots reveals that protein complexes can change their conformation in a cell-type-dependent manner to regulate specific gene expression programs leading to precise specification and ...
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Protocol
Analysis of a Plant Transcriptional Regulatory Network Using Transient Expression Systems
In plant biology, transient expression systems have become valuable approaches used routinely to rapidly study protein expression, subcellular localization, protein-protein interactions, and transcriptional ac...