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  1. No Access

    Article

    A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris

    Phytase is an additive in animal feed that degrades phytic acid in plant material, reducing feeding costs, and pollution from fecal phosphorus excretion. A multistrategy approach was adopted to improve the exp...

    Yuankun Helian, Yuanming Gai, Huan Fang… in Journal of Industrial Microbiology & Biote… (2020)

  2. Article

    Open Access

    Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

    Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, produ...

    **gqi Chen, Gang Fu, Yuanming Gai, ** Zheng, Dawei Zhang… in Microbial Cell Factories (2015)

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  3. No Access

    Article

    Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein

    α-Amylase was used as a heterologous model protein to investigate the effects of promoters, signal peptides and over-expression of an extra-cytoplasmic molecular chaperone, PrsA lipoprotein, on enhancing the s...

    **gqi Chen, Yuanming Gai, Gang Fu, Wenjuan Zhou, Dawei Zhang… in Biotechnology Letters (2015)