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Open AccessLong- and short-read RNA sequencing from five reproductive organs of boar
The production of semen in boars involves multiple reproductive glands, including the testis (Tes), epididymis (Epi), vesicular gland (VG), prostate gland (PG), and bulbourethral gland (BG). However, previous ...
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Article
Open AccessscRNASequest: an ecosystem of scRNA-seq analysis, visualization, and publishing
Single-cell RNA sequencing is a state-of-the-art technology to understand gene expression in complex tissues. With the growing amount of data being generated, the standardization and automation of data analysi...
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Article
Open AccessLSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei
Epigenetic reprogramming of the parental genome is essential for zygotic genome activation and subsequent embryo development in mammals. Asymmetric incorporation of histone H3 variants into the parental genome...
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Article
Open AccessAmniotes co-opt intrinsic genetic instability to protect germ-line genome integrity
Unlike PIWI-interacting RNA (piRNA) in other species that mostly target transposable elements (TEs), >80% of piRNAs in adult mammalian testes lack obvious targets. However, mammalian piRNA sequences and piRNA-...
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Article
Open AccessGenomic signatures associated with maintenance of genome stability and venom turnover in two parasitoid wasps
Parasitoid wasps are rapidly develo** as a model for evolutionary biology. Here we present chromosomal genomes of two Anastatus wasps, A. japonicus and A. fulloi, and leverage these genomes to study two fundame...
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Article
Open AccessThe birth of piRNAs: how mammalian piRNAs are produced, originated, and evolved
PIWI-interacting RNAs (piRNAs), small noncoding RNAs 24–35 nucleotides long, are essential for animal fertility. They play critical roles in a range of functions, including transposable element suppression, ge...
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Article
Open AccessCoupled protein synthesis and ribosome-guided piRNA processing on mRNAs
PIWI-interacting small RNAs (piRNAs) protect the germline genome and are essential for fertility. piRNAs originate from transposable element (TE) RNAs, long non-coding RNAs, or 3´ untranslated regions (3´UTRs)...
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Article
Open AccessGenome of the pincer wasp Gonatopus flavifemur reveals unique venom evolution and a dual adaptation to parasitism and predation
Hymenoptera comprise extremely diverse insect species with extensive variation in their life histories. The Dryinidae, a family of solitary wasps of Hymenoptera, have evolved innovations that allow them to hun...
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Open AccessSingle-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm
Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown to impact offspring phenotypes, our knowledge of the sperm transcriptome, especially the composition of long RNAs, has been ...
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Author Correction: Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors
PIWI-interacting RNAs (piRNAs) are a class of small non-coding RNAs essential for fertility. In adult mouse testes, most piRNAs are derived from long single-stranded RNAs lacking annotated open reading frames ...