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Article
Open AccessCorrelative light and electron microscopic observation of calcium phosphate particles in a mouse kidney formed under a high-phosphate diet
Calcium phosphate forms particles under excessive urinary excretion of phosphate in the kidney. While the formation of calcium phosphate particles (CaPs) has been implicated in the damage to renal tubular cell...
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Article
Deep learning-based point-scanning super-resolution imaging
Point-scanning imaging systems are among the most widely used tools for high-resolution cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution, speed, sample preservation ...
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Protocol
Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells
It is now well known that there are many neuron subtypes in the brain. For instance, a few subtypes of pyramidal cell in each layer and 10 or more non-pyramidal cell subtypes are found in the neocortex. Their ...
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Article
Open AccessUNI-EM: An Environment for Deep Neural Network-Based Automated Segmentation of Neuronal Electron Microscopic Images
Recently, there has been rapid expansion in the field of micro-connectomics, which targets the three-dimensional (3D) reconstruction of neuronal networks from stacks of two-dimensional (2D) electron microscopy...
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Article
Open AccessA carbon nanotube tape for serial-section electron microscopy of brain ultrastructure
Automated tape-collecting ultramicrotomy in conjunction with scanning electron microscopy (SEM) is a powerful approach for volume electron microscopy and three-dimensional neuronal circuit analysis. Current ta...
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Article
Input integration around the dendritic branches in hippocampal dentate granule cells
Recent studies have shown that the dendrites of several neurons are not simple translators but are crucial facilitators of excitatory postsynaptic potential (EPSP) propagation and summation of synaptic inputs ...
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Article
New insights into the classification and nomenclature of cortical GABAergic interneurons
A feature-based classification and agreed-upon nomenclature of GABAergic interneurons of the cerebral cortex is much needed but currently lacking.
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Chapter and Conference Paper
Functional Significance of Rall’s Power of Three Halves Law in Cortical Nonpyramidal Cells
Neurons receive thousands of synaptic inputs onto their dendrites and soma, and spatially and temporally integrate these inputs to produce appropriate output in the form of action potentials generated in axons...
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Article
Open AccessConserved properties of dendritic trees in four cortical interneuron subtypes
Dendritic trees influence synaptic integration and neuronal excitability, yet appear to develop in rather arbitrary patterns. Using electron microscopy and serial reconstructions, we analyzed the dendritic tre...
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Article
Structural basis for the role of inhibition in facilitating adult brain plasticity
Chen and colleagues show that visual deprivation drives structural remodeling of the dendritic branch tips of cortical layer 2/3 interneurons in an input-specific manner. Neighboring pyramidal cells show a cor...
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Chapter and Conference Paper
Local Circuit Neurons in the Frontal Cortex and the Neostriatum
We have compared the cellular organizations of local circuit neurons in frontal cortex and neostriatum of rats using whole-cell current-clamp recording in vitro combined with intracellular staining and immunohist...
