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Article
Open AccessSesamolin serves as an MYH14 inhibitor to sensitize endometrial cancer to chemotherapy and endocrine therapy via suppressing MYH9/GSK3β/β-catenin signaling
Endometrial cancer (EC) is one of the most common gynecological cancers. Herein, we aimed to define the role of specific myosin family members in EC because this protein family is involved in the progression o...
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Article
Open AccessEfficient strategy for introducing large and multiple changes in plasmid DNA
While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligat...
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Article
Open AccessAFEAP cloning: a precise and efficient method for large DNA sequence assembly
Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembl...
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Article
Open AccessA restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...