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Article
Open AccessCharacterization of a fluorescence imaging probe that exploits metabolic dependency of ovarian clear cell carcinoma
The purpose of this study is to clarify the metabolic dependence of ovarian clear cell carcinoma (CCC) by comparing normal tissues and to examine the applicability of fluorescence imaging probe to exploit thes...
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Article
Open AccessDiscovery of a cystathionine γ-lyase (CSE) selective inhibitor targeting active-site pyridoxal 5′-phosphate (PLP) via Schiff base formation
D,L-Propargylglycine (PAG) has been widely used as a selective inhibitor to investigate the biological functions of cystathionine γ-lyase (CSE), which catalyzes the formation of reactive sulfur species (RSS). How...
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Article
Open AccessDevelopment of pathway-oriented screening to identify compounds to control 2-methylglyoxal metabolism in tumor cells
Controlling tumor-specific alterations in metabolic pathways is a useful strategy for treating tumors. The glyoxalase pathway, which metabolizes the toxic electrophile 2-methylglyoxal (MG), is thought to contr...
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Article
Open AccessRapid imaging of thymoma and thymic carcinoma with a fluorogenic probe targeting γ-glutamyltranspeptidase
In recent years, thoracoscopic and robotic surgical procedures have increasingly replaced median sternotomy for thymoma and thymic carcinoma. In cases of partial thymectomy, the prognosis is greatly improved b...
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Article
Open AccessAuthor Correction: Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration
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Chapter
Development of Novel Fluorescent Probes: Rapid Intraoperative Visualization of Microcarcinoma by Local Application of Chemical Fluorescence Probes
Fluorescence probes are molecular tools that change their fluorescent property dramatically by specific reactions to the target molecule. In recent years, fluorescent probes have been widely used to observe th...
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Article
Open AccessSenescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration
Tissue regeneration requires coordination between resident stem cells and local niche cells1,2. Here we identify that senescent cells are integral components of the skeletal muscle regenerative niche that repress...
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Article
Open AccessRapid imaging of lung cancer using a red fluorescent probe to detect dipeptidyl peptidase 4 and puromycin-sensitive aminopeptidase activities
Rapid identification of lung-cancer micro-lesions is becoming increasingly important to improve the outcome of surgery by accurately defining the tumor/normal tissue margins and detecting tiny tumors, especial...
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Article
Open AccessDevelopment of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements
Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Develop...
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Article
Open AccessRapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
Fluorescence imaging of tumours facilitates rapid intraoperative diagnosis. Thus far, a promising activatable fluorescence probe for hepatocellular carcinoma (HCC) has not been developed. Herein, the utility o...
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Article
Open Accessβ-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
Diagnosis of peritoneal metastasis in gastric cancer (GC) is essential for determining appropriate therapeutic strategies and avoiding non-essential laparotomy or gastrectomy. Recently, a variety of activatabl...
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Protocol
Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity
Fluorescence (FL)-guided detection of cancer is one of the most promising approaches to achieve intraoperative assessment of surgical margins. Enzymes, such as aminopeptidase, carboxypeptidase, and glycosidase...
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Article
ɤ-glutamyl hydroxymethyl rhodamine green fluorescence as a prognostic indicator for lung cancer
ɤ-glutamyltranspeptidase is an enzyme expressed in various malignancies including lung cancer. It rapidly activates non-fluorescent ɤ-glutamyl hydroxymethyl rhodamine green to highly fluorescent hydroxymethyl ...
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Article
Open AccessOn-Site Monitoring of Postoperative Bile Leakage Using Bilirubin-Inducible Fluorescent Protein
Bile leakage is the most common postoperative complication associated with hepatobiliary and pancreatic surgery. Until now, however, a rapid, accurate diagnostic method for monitoring intraoperative and postop...
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Article
Open AccessCalciprotein particle-induced cytotoxicity via lysosomal dysfunction and altered cholesterol distribution in renal epithelial HK-2 cells
Dietary phosphate overload induces chronic kidney disease (CKD), and calciprotein particles (CPPs), a form of nanoparticle comprising calcium phosphate and serum proteins, has been proposed to cause renal toxi...
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Article
Open AccessA novel method for assessing the renal biopsy specimens using an activatable fluorescent probe
Gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) is an activatable fluorescent probe that can be activated by γ-glutamyltranspeptidase (GGT). The expression of GGT in the kidney, which is one of the ma...
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Article
Open AccessMolecular design strategy of fluorogenic probes based on quantum chemical prediction of intramolecular spirocyclization
Fluorogenic probes are essential tools for real-time visualization of dynamic intracellular processes in living cells, but so far, their design has been largely dependent on trial-and-error methods. Here we pr...
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Article
Open AccessDetection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV
It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green ...
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Chapter and Conference Paper
Development of Novel Fluorogenic Probes for Realizing Rapid Intraoperative Multi-color Imaging of Tiny Tumors
We found that hydroxymethyl rhodamine green (HMRG) strongly fluoresces, while mono-amidated HMRG derivatives are colorless and non-fluorescent due to the preferred spirocyclized structure in aqueous solution a...
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Protocol
Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties
Super-resolution fluorescence imaging by single-molecule localization microscopy (SMLM) has paved the way for a better understanding of cellular structures and processes at the molecular scale. Since SMLM reli...
