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Chapter
Solid-phase synthesis of caged peptides containing photolabile derivatives of aspartic acid
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Article
Sustainable and practical degradation of intact chicken feathers by cultivating a newly isolated thermophilic Meiothermus ruber H328
The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermo...
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Article
High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides
Post-translational modifications (PTMs) of proteins are important in the activation, localization, and regulation of protein function in vivo. The usefulness of electron capture dissociation (ECD) and electron...
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Article
Desorption/Ionization Efficiency of Peptides Containing Disulfide Bonds in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
In order to elucidate the role of desorption/ionization efficiency of peptides in MALDI-MS, we focused on peptides with disulfide bonds, which form a rigid tertiary structure. We synthesized seven sets of pept...
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Article
Efficient secreted production of (R)-3-hydroxybutyric acid from living Halomonas sp. KM-1 under successive aerobic and microaerobic conditions
Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storag...
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Article
Extraordinary denaturant tolerance of keratinolytic protease complex assemblies produced by Meiothermus ruber H328
A moderately thermophilic bacterial strain, Meiothermus ruber H328, can efficiently solubilize intact chicken feathers by aerobic cultivation at 55 °C for 6 days. The keratinolytic proteases extracellularly secre...
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Protocol
Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepods
Secreted luciferases isolated from copepod crustaceans are frequently used for nondisruptive reporter-gene assays, such as the continuous, automated and/or high-throughput monitoring of gene expression in livi...
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Article
Sensing of ozone based on its quenching effect on the photoluminescence of CdSe-based core-shell quantum dots
Thin films of CdSe-based core-shell type quantum dots (CdSe/CdZnS, CdSe/ZnS and CdSeTe/ZnS) deposited on glass substrate were found to undergo a reversible change in photoluminescence (PL) on exposure to ozone...
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Article
Open AccessIsolation of biologically active peptides from the venom of Japanese carpenter bee, Xylocopa appendiculata
Mass spectrometry-guided venom peptide profiling is a powerful tool to explore novel substances from venomous animals in a highly sensitive manner. In this study, this peptide profiling approach is successfull...
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Article
Development of Technologies for Sensing Ozone in Ambient Air
Ozone (O3) gas is widely used as a strong oxidizing agent for many purposes, such as the decomposition/removal of organic contaminants and photoresist, and the deodorization/disinfection of air and water. However...
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Article
Photoluminescent Ozone Sensor with Enhanced Sensitivity by Using CdSe/ZnS Quantum Dots Modified with Gold and Platinum
We report photoluminescence-based ozone sensing using composite films composed of gold or platinum and red-emitting CdSe/ZnS core-shell quantum dots. The sensing efficiency of quantum dots is enhanced by the a...
