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Article
Open AccessKalium channelrhodopsins effectively inhibit neurons
The analysis of neural circuits has been revolutionized by optogenetic methods. Light-gated chloride-conducting anion channelrhodopsins (ACRs)—recently emerged as powerful neuron inhibitors. For cells or sub-n...
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Article
Open AccessVisualization of accessible cholesterol using a GRAM domain-based biosensor
Cholesterol is important for membrane integrity and cell signaling, and dysregulation of the distribution of cellular cholesterol is associated with numerous diseases, including neurodegenerative disorders. Wh...
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Article
Open AccessRegulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites
Abnormal distribution of cellular cholesterol is associated with numerous diseases, including cardiovascular and neurodegenerative diseases. Regulated transport of cholesterol is critical for maintaining its p...
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Article
Open AccessPDZD-8 and TEX-2 regulate endosomal PI(4,5)P2 homeostasis via lipid transport to promote embryogenesis in C. elegans
Different types of cellular membranes have unique lipid compositions that are important for their functional identity. PI(4,5)P2 is enriched in the plasma membrane where it contributes to local activation of key ...
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Chapter
Endoplasmic Reticulum – Plasma Membrane Crosstalk Mediated by the Extended Synaptotagmins
The endoplasmic reticulum (ER) possesses multiplicity of functions including protein synthesis, membrane lipid biogenesis, and Ca2+ storage and has broad localization throughout the cell. While the ER and most ot...
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Article
Control of plasma membrane lipid homeostasis by the extended synaptotagmins
Acute metabolic changes in plasma membrane (PM) lipids, such as those mediating signalling reactions, are rapidly compensated by homeostatic responses whose molecular basis is poorly understood. Here we show t...
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Article
Structure of a lipid-bound extended synaptotagmin indicates a role in lipid transfer
Several proteins localized at membrane contact sites contain an SMP domain, which has been proposed to act as a lipid-binding module; here, the crystal structure of a fragment of the extended synaptotagmin 2 p...
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Article
Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α2δ subunit UNC-36
In C. elegans, the unc-2 gene encodes the pore-forming subunit of a voltage-gated Ca2+ channel (CaV2) involved in neurotransmitter release. Here, the authors identify a protein, CALF-1, that regulates the presyna...
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Article
Open AccessLeft-right olfactory asymmetry results from antagonistic functions of voltage-activated calcium channels and the Raw repeat protein OLRN-1 in C. elegans
The left and right AWC olfactory neurons in Caenorhabditis elegans differ in their functions and in their expression of chemosensory receptor genes; in each animal, one AWC randomly takes on one identity, designa...
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Article
Cdk5-dependent regulation of glucose-stimulated insulin secretion
Tight glycemic control in individuals with diabetes mellitus is essential to prevent or delay its complications1. Present treatments to reduce hyperglycemia mainly target the ATP-sensitive K+ (KATP) channel of pa...
