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Article
Phthirus pubis (Anoplura: Phthiridae) infestation of the palpebral margin and scalp hair in an infant
This paper reports a rare case of Phthirus pubis infestation of the palpebral margin and scalp hair in an 8-month-old infant. Under the microscope, the lice were observed to have a short and wide, crab-like shape...
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Article
Morphological and Molecular Identification of Oestrus ovis (Diptera: Oestridae) Larvae Collected from a Chinese Patient with Conjunctival Myiasis
Human conjunctival myiasis, which is often misdiagnosed or missed clinically, is commonly caused by Oestrus ovis larvae. Here, pathogenic identification was performed for two maggots collected from a patient from...
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Article
De novo transcriptome sequencing and functional annotation of Demodex canis
Demodex canis is an important parasitic mite causing skin lesions in dogs. However, molecular studies on this species are limited because of the lack of transcriptomic data. To obtain functional genes of D. canis
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Article
Molecular Identification, Transcriptome Sequencing and Functional Annotation of Pulex irritans
Pulex irritans are vectors of various zoonotic pathogens. However, molecular studies on P. irritans and flea-borne diseases are limited due to the lack of molecular data. This study aimed to conduct transcriptome...
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Article
Open AccessDivergent domains of 28S ribosomal RNA gene: DNA barcodes for molecular classification and identification of mites
The morphological and molecular identification of mites is challenging due to the large number of species, the microscopic size of the organisms, diverse phenotypes of the same species, similar morphology of d...
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Article
De Novo RNA-seq and Functional Annotation of Haemaphysalis longicornis
Haemaphysalis longicornis (Neumann) is a hematophagous tick widely distributed in northern China. It not only causes enormous economic loss to animal husbandry, but also as a vector and reservoir of various zoono...
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Article
De novo transcriptome sequencing and differential gene expression analysis of two parasitic human Demodex species
Demodex are among the tiniest organisms in Acari and are important mammalian parasites. However, differences in pathogenicity between two human parasites, Demodex folliculorum and Demodex brevis, remain unknown. ...
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Article
De novo RNA-seq and functional annotation of Ornithonyssus bacoti
Ornithonyssus bacoti (Hirst) (Acari: Macronyssidae) is a vector and reservoir of pathogens causing serious infectious diseases, such as epidemic hemorrhagic fever, endemic typhus, tularemia, and leptospirosis. It...
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Article
DNA barcoding for molecular identification of Demodex based on mitochondrial genes
There has been no widely accepted DNA barcode for species identification of Demodex. In this study, we attempted to solve this issue. First, mitochondrial cox1-5′ and 12S gene fragments of Demodex folloculorum, D...
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Article
cDNA library construction of two human Demodex species
The research of Demodex, a type of pathogen causing various dermatoses in animals and human beings, is lacking at RNA level. This study aims at extracting RNA and constructing cDNA library for Demodex. First, P. ...
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Article
Association study of Demodex bacteria and facial dermatoses based on DGGE technique
The role of bacteria is unclear in the facial skin lesions caused by Demodex. To shed some light on this issue, we conducted a case-control study comparing cases with facial dermatoses with controls with healthy ...
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Article
De novo RNA-Seq and functional annotation of Sarcoptes scabiei canis
The transcriptomic data of Sarcoptes is still lacking in the public database due to the difficulty in extracting high-quality RNA from tiny mites with thick chitin. In this study, total RNA was extracted from liv...
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Article
Improvement on the extraction method of RNA in mites and its quality test
To solve the long-existing difficult problems in extracting RNA and constructing a complementary DNA (cDNA) library for trace mites, we conducted a further comparative experiment among three RNA extraction met...
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Article
Constructing and detecting a cDNA library for mites
RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is...
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Article
Population identification of Sarcoptes hominis and Sarcoptes canis in China using DNA sequences
There has been no consistent conclusion on whether Sarcoptes mites parasitizing in humans and animals are the same species. To identify Sarcoptes (S.) hominis and S. canis in China, gDNA was extracted from indivi...
