62 Result(s)
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Article
A molecular beacon design for a colorimetric loop-mediated isothermal amplification assay
In this study, a molecular beacon (MB) was designed for colorimetric loop-mediated isothermal amplification (cLAMP). The length of complementary bases on the MB, guanine and cytosine content (GC content), and ...
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Article
Open AccessDynamic three-dimensional structures of a metal–organic framework captured with femtosecond serial crystallography
Crystalline systems consisting of small-molecule building blocks have emerged as promising materials with diverse applications. It is of great importance to characterize not only their static structures but al...
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Article
Phonozen-mediated photodynamic therapy comparing two wavelengths in a mouse model of peritoneal carcinomatosis
This study assessed the therapeutic efficacy of intraperitoneal photodynamic therapy (PDT) using photosensitizer activation at two different wavelengths, 405 and 664 nm, in a mouse model of peritoneal carcinom...
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Article
Rapid detection of Shiga-toxin-producing Escherichia coli O157:H7 based on a colorimetric loop-mediated isothermal amplification (cLAMP) assay using a molecular beacon paired with HRPzyme
Contamination by Escherichia coli O157:H7 is considered a threat in the livestock and food industries. Therefore, it is necessary to develop methods for the convenient and rapid detection of Shiga-toxin-producing...
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Article
Mesoporous platinum nanoparticles as a peroxidase mimic for the highly sensitive determination of C-reactive protein
The generation of a mesoporous structure in platinum nanoparticles can effectively enhance physical and chemical properties. In this study, mesoporous platinum nanoparticles (MPNs) were synthesized by a soft t...
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Article
Colorimetric detection of norovirus by helicase-dependent amplification method based on specific primers integrated with HRPzyme
Noroviruses (NoVs) are the most common causes of epidemic gastroenteritis, responsible for at least 50% of all gastroenteritis outbreaks worldwide and significant causes of foodborne illness. In the USA, appro...
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Article
In situ multiscale probing of the synthesis of a Ni-rich layered oxide cathode reveals reaction heterogeneity driven by competing kinetic pathways
Nickel-rich layered oxides are envisaged as key near-future cathode materials for high-energy lithium-ion batteries. However, their practical application has been hindered by their inferior cycle stability, wh...
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Article
Nanoceria-based lateral flow immunoassay for hydrogen peroxide-free colorimetric biosensing for C-reactive protein
During the recent several decades, lateral flow immunoassay (LFIA) constructed with gold nanoparticle (AuNP) has been widely utilized to conveniently detect target analyte. However, AuNP-based LFIA has limitat...
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Protocol
Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues
New methods to analyze cells and tissues in ambient condition without any harsh chemical fixation or physical freezing and drying are summarized in this report. The first approach, an atmospheric pressure mass...
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Article
Production of Tyrian purple indigoid dye from tryptophan in Escherichia coli
Tyrian purple, mainly composed of 6,6'-dibromoindigo (6BrIG), is an ancient dye extracted from sea snails and was recently demonstrated as a biocompatible semiconductor material. However, its synthesis remains...
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Article
Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization
Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the ...
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Article
Maintenance of viability and proliferation of 3T3 cell aggregates incorporating fibroin microspheres into cultures
This study investigated whether micron-sized microspheres can be used as dispersed scaffolds where anchorage-dependent cells can proliferate and survive in suspension culture. Aggregates of murine 3T3 cells in...
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Article
Comparison of Fabrication Methods Based on Nanoimprinting Lithography for Plasmonic Color Filter Fabrication
The angle-variable tunable optical filter was strictly fabricated by two strategies of nanoimprint-coupled metal nanopatterning with improved cost-effectiveness and accessibility. The tunable optical propertie...
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Article
Targeting the interaction of AIMP2-DX2 with HSP70 suppresses cancer development
A tumorigenic factor, AIMP2 lacking exon 2 (AIMP2-DX2), is often upregulated in many cancers. However, how its cellular level is determined is not understood. Here, we report heat-shock protein HSP70 as a crit...
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Article
Quantitative imaging of magnetic field distribution using a pyrene-based magnetosensing exciplex fluorophore
Quantitative imaging of magnetic field distribution was carried out using a pyrene-based magnetosensing exciplex fluorophore, pyrene-(CH2)12-O-(CH2)2-N,N-dimethylaniline (Py-12-O-2-DMA), on a conventional fluores...
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Article
Engineering of an oleaginous bacterium for the production of fatty acids and fuels
Production of free fatty acids (FFAs) and derivatives from renewable non-food biomass by microbial fermentation is of great interest. Here, we report the development of engineered Rhodococcus opacus strains produ...
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Article
Polarization- and wavelength-dependent defocused scattering imaging of single gold nanostars with multiple long branches
The scattering properties of gold nanostars (AuNSs) are not completely understood. Furthermore, there have been no studies on the dark-field (DF) scattering patterns of a AuNS in defocused DF microscopy. Herei...
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Article
Immunomodulatory Effects of Monascus spp.-Fermented Sacccharina japonica Extracts on the Cytokine Gene Expression of THP-1 Cells
The immunomodulatory effects of Monascus-fermented Saccharina japonica extract on anti- and pro-inflammatory cytokines gene expression of THP-1 cells were evaluated. Extracts of fermented samples showed higher ph...
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Article
Complete reconstitution of the diverse pathways of gentamicin B biosynthesis
Gentamicin B (GB), a valuable starting material for the preparation of the semisynthetic aminoglycoside antibiotic isepamicin, is produced in trace amounts by the wild-type Micromonospora echinospora. Though the ...
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Article
Monitoring of post-mortem changes of saliva N-glycosylation by nano LC/MS
The estimation of post-mortem interval (PMI) is a crucial part for investigations of crime and untimely deaths in forensic science. However, standard methods of PMI estimation are easily confounded by extenuat...
