18 Result(s)
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Protocol
Measurements of Oxygen Evolution in Photosynthesis
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We...
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Protocol
Liquid-Phase Measurements of Photosynthetic Oxygen Evolution
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We ...
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Article
Open AccessIsolation of monomeric photosystem II that retains the subunit PsbS
Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was pos...
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Article
Open AccessArabidopsisplants grown in the field and climate chambers significantly differ in leaf morphology and photosystem components
Plants exhibit phenotypic plasticity and respond to differences in environmental conditions by acclimation. We have systematically compared leaves of Arabidopsis thaliana plants grown in the field and under contr...
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Protocol
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis
For many studies regarding important chloroplast processes such as oxygenic photosynthesis, fractionation of the total chloroplast proteome is a necessary first step. Here, we describe a method for isolating t...
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Article
A novel extended family of stromal thioredoxins
Thioredoxins play key regulatory roles in chloroplasts by linking photosynthetic light reactions to a series of plastid functions. In addition to the established groups of thioredoxins, f, m, x, and y, novel p...
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Chapter and Conference Paper
Thioredoxin Interactions of the Chloroplast Lumen of Arabidopsis thaliana Indicate a Redox Regulation of the Xanthophyll Cycle
Redox signalling via thioredoxins plays central roles in the light-mediated regulation of metabolic pathways of the chloroplast. Recent observations indicate strongly that thiotransduction pathways not only ta...
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Article
The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II
The PsbP-like protein of the cyanobacterium Synechocystis sp. PCC 6803 is a peripheral component of Photosystem II, located at the lumenal side of the thylakoid membrane. Removal of this protein leads to decrease...
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Article
Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803
A recent proteomic analysis of the thylakoid lumen of Arabidopsis thaliana revealed the presence of several PsbP-like proteins, and a homologue to this gene family was detected in the genome of the cyanobacterium...
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Article
The proteome of the chloroplast lumen of higher plants
Recent research in proteomics of the higher plant chloroplast has achieved considerable progress and added to our knowledge of lumenal chloroplast proteins. This work shows that chloroplast lumen has its own s...
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Article
Update on chloroplast proteomics
Currently, relatively few proteomics studies of chloroplast have been published, but the field has just started emerging and is likely to develop more rapidly in the future. While the complex membrane structur...
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Article
Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants
The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles b...
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Article
Characterisation of an Arabidopsis thaliana cDNA encoding a novel thylakoid lumen protein imported by the ΔpH-dependent pathway
An Arabidopsis thaliana (L.) Heynh. cDNA encoding a novel 16-kDa protein (P16) of the chloroplast thylakoid lumen has been characterised. The function of the protein is unknown but it shares some sequence simila...
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Chapter
PsbX Protein from Photosystem II in Higher Plants: Localization and Gene Regulation
Photosystem H complex (PSII) of higher plants consists of more than 25 different subunits (for reviews, see ref. 1 and 2). The D1 and D2 proteins have been found to bind most, if not all of the cofactors neede...
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Chapter
PsbX Protein from Photosystem II in Higher Plants: Localization and Gene Regulation
Photosystem II complex (PSII) of higher plants consists of more than 25 different subunits (for reviews, see ref. 1 and 2). The D1 and D2 proteins have been found to bind most, if not all of the cofactors need...
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Article
Compositional and topological studies of the PsbW protein in spinach thylakoid membrane
The lateral distribution and transversal orientation of the nuclear encoded PsbW protein (psbW gene product) has been investigated. The main part (80%) of the PsbW protein was found in the grana region of the ...
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Article
Photosynthetic water oxidation: The protein framework
Approximately 20 protein subunits are associated with the PS II complex, not counting subunits of peripheral light-harvesting antenna complexes. However, it is not yet established which proteins specifically a...
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Chapter
Hydrogen Peroxide Production in Photosystem II Preparations
A photosystem II (PS-II) associated hydrogen peroxide production has recently been reported (1,2). It was found that when inside-out thylakoids or detergent prepared PS-II particles from spinach were illuminat...
