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Article
Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species
The role of the plasma membrane (PM) H+-ATPase (E.C. 3.6.1.3) in the plant’s response to salt stress was studied in the perennial leguminosae forage Medicago arborea L. and its close relative Medicago citrina (Fo...
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Article
Immunolocalization of plasma-membrane H+-ATPase and tonoplast-type pyrophosphatase in the plasma membrane of the sieve element-companion cell complex in the stem of Ricinus communis L.
Plasma-membrane-located primary pumps were investigated in the sieve element (SE)-companion cell complex in the transport phloem of 2-week-old stems of Ricinus communis L. and, for comparison, in stems of Cucurb...
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Article
Molecular and physiological characterisation of a 14-3-3 protein from lily pollen grains regulating the activity of the plasma membrane H+ ATPase during pollen grain germination and tube growth
A 14-3-3 protein has been cloned and sequenced from a cDNA library constructed from mRNAs of mature pollen grains of Lilium longiflorum Thunb. Monoclonal antibodies (MUP 5 or MUP 15) highly specific against 14-3...
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Article
Phytotropin-binding sites and auxin transport in Cucurbita pepo: evidence for two recognition sites
Two properties of phytotropins, their ability to bind to 1-N-naphthylphthalamic acid (NPA) receptors located on microsomal vesicles isolated from Cucurbita pepo L. hypocotyls, and to stimulate auxin (indol-3-yl a...
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Article
Membranes markers in highly purified clathrin-coated vesicles from Cucurbita hypocotyls
The two plasma-membrane (PM) markers: 1,3-β-glucan synthase and naphthylphthalamic-acid-binding capacity are localized in two peaks of activity on isopycnic Ficoll/D2O gradients prepared from a zucchini (Cucurbit...
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Article
Bisulfite interacts with binding sites of the auxin-transport inhibitor N-1-naphthylphthalamic acid
The affinity of the auxin-transport inhibitor N-1-naphthylphthalamic acid (NPA) for membrane particles as well as for solubilized binding sites from Cucurbita pepo L. hypocotyls was reduced by low concentrations ...
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Article
Fractionation of particulate material from maize coleoptile homogenates with polyethylene glycol
Polyethyleneglycol (PEG) has been used to sediment particulate material from maize coleoptile homogenates at low centrifugal forces. The resuspended sediments were used for N-1-naphthylphthalamic acid (NPA)-bi...
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Article
Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates
The association of NPA with particulate material from corn coleoptile homogenates and the dissociation of the complexes formed are characterized. Using a filtration method, a single rate constant for the assoc...
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Article
Communication between Cells of Different Type
MANY types of cells have membrane junctions so organized as to allow the passage of molecules from the interior of one cell to another with little loss to the outside1–6. At least in some cases, a large part if n...