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Article
Open AccessGORAB scaffolds COPI at the trans-Golgi for efficient enzyme recycling and correct protein glycosylation
COPI is a key mediator of protein trafficking within the secretory pathway. COPI is recruited to the membrane primarily through binding to Arf GTPases, upon which it undergoes assembly to form coated transport...
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Article
Open AccessCharacterization of a Propionibacterium acnes Surface Protein as a Fibrinogen-Binding Protein
Propionibacterium acnes (P. acnes) is a major skin-associated bacterium that was long considered commensal, until several studies revealed it to be an opportunistic pathogen. We investiga...
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Article
Open AccessRadiation-induced changes in the glycome of endothelial cells with functional consequences
As it is altered by ionizing radiation, the vascular network is considered as a prime target in limiting normal tissue damage and improving tumor control in radiation therapy. Irradiation activates endothelial...
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Article
Open AccessRelationship between the sialylation level of anti-proteinase 3 antibodies and the activity of granulomatosis with polyangiitis (Wegener’s disease)
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Protocol
Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry
Glycosylation represents the most common of all known protein post-translational modifications. Carbohydrates can modulate the biological functions of a glycoprotein, protect a protein against hydrolysis via p...
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Article
Analysis of protein glycosylation by mass spectrometry
We present a detailed protocol for the structural analysis of protein-linked glycans. In this approach, appropriate for glycomics studies, N-linked glycans are released using peptide N-glycosidase F and O-link...
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Article
FAB-MS characterization of sialyl Lewisx determinants on polylactosamine chains of human airway mucins secreted by patients suffering from cystic fibrosis or chronic bronchitis
Although a large body of structural data exists for bronchial mucins from cystic fibrosis (CF) and chronic bronchitis (CB) patients, little is known about terminal structures carried on poly-N-acetyllactosamine a...
